摘要
以何首乌茎尖、茎段为外植体,经体细胞胚发生途径,进行胚性愈伤组织诱导、体细胞胚的诱导、植株再生的研究,并采用临时压片法对体细胞胚的发育过程进行观察。结果表明愈伤组织诱导最适培养基为MS+6-BA 2.0 mg/L+NAA 0.5 mg/L,体细胞胚诱导最适培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L。将产生的体细胞胚首先接种于MS基本培养基使其充分发育后转入MS+6-BA 2.0 mg/L培养基中诱导出芽,出芽率高于直接采用MS+6-BA 2.0 mg/L培养基诱导。体细胞胚的发育过程是首先在愈伤组织表面形成许多瘤状突起即胚性细胞团,胚性细胞团继续发育成球形胚、盾形胚,球形胚、盾形胚成熟后发育成植株。
Taking the slice of Fleece flower root as explants, the regenerated way of plant was selected for the study of somatic embryogensis. MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.5mg/L was the best culture medium for callus induction. MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.2 mg/L was the best culture medium for somatic embryo induction. After that there were three steps to complete the culture. First, the regenerated somatic embryo were cultured in the MS medium to grow for enoughtime. Second, the growthful somatic embryo was cultured in the MS ＋ 6-BA 2.0 mg/L to induce buds. Third, the regen- erated plants were obtained in the MS medium. The appearance and growth of somatic embryo were observed through paraffin slices and press slices. Histological observations showed that parenchyma ceils first experienced dedifferentiation and followed with callus-forming, ensued with embryogenic cell masses which are like a tubercular structure. And these embryogenic cell masses experienced globular, scutellate stages and developed into whole plantlets.
出处
《激光生物学报》
CAS
CSCD
2010年第2期229-234,共6页
Acta Laser Biology Sinica
基金
国家重大转基因专项(2008ZX08010-001)
安徽省教育厅项目(KJ2008B062)
关键词
何首乌
胚性愈伤组织
体细胞胚
Polygonum multiflorum Thunb
embryogenic callus
somatic embryo
