摘要
目的 :研究大鼠老化进程中主要晶体蛋白相对含量的时相变化与晶体老化的关系。方法 :常规饲养不同年龄SD大鼠 (出生后 1d、8d、2周、8周、8个月及 1.5年 ) ;提取水溶性晶体蛋白 ,双向电泳 (等电聚焦 /SDS -聚丙烯酰胺凝胶电泳 ,IEF/SDS -PAGE)得到大鼠晶体蛋白电泳图形 ;考马斯亮蓝染色后扫描蛋白图形 ,分别确认各主要晶体蛋白组分并测定其相对含量百分比组成。结果 :(1)所测各组大鼠 18种主要晶体蛋白组分中 ,7种晶体蛋白相对含量呈渐进性时相改变 ,但总水溶性晶体蛋白水平未见明显变化。 (2 )晶体蛋白相对含量时相改变主要有 4种形式 :升高 (βB4 、αB2 、αA2 、βA1)、降低 (β7、β8、γ2 ,3 、γ5 ,6)、大致保持稳定 (βA3 、βB5 )及无规则性改变。 (3 )晶体蛋白 βB4/αA2比值在大鼠老化进程中呈渐进性升高。结论
AIM: To study the correlation between phasic change of the relative quantity of major crystallins with aging in rats. METHODS: Six groups of SD ra ts (age 1 d, 8 d,2 weeks,8 weeks,8 months and 1.5 years) were raised routine ly. Water soluble crystallins were extracted and separated by two-dimensional polyacrylamide gel electrophoresis. After comassize blue staining,the crystallins patterns were sc anned and analyzed. RESULTS: (1) Out of the eighteen water solu ble major rat cr ystallins tested in each group, seven showed gradual phasic changes in relative quantity of crystallins, but there were no significant changes in total quantity of water soluble crystallins. (2) Phasic changes in these crystallins pres ented four different patterns: increasing (βB 4?αB 2?αA 2?βA 1), decreasi ng (β 7?β 8?γ 2,3 ?γ 5,6 ),relatively stable(βA 3?βB 5) , and irregular. (3) The ratio of βB 4 /αA 2 increased gradually with the r at aging process. CONCLUSION: T he gradual phasic changes in relative quantity of crystallins reflect the aging status of rat crystalline.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2004年第1期22-26,共5页
Chinese Journal of Pathophysiology
