摘要
以甘蓝为母本与白菜杂交,授粉后采不同时期的子房用苯胺兰法染色,在荧光显微镜下观察花粉在柱头上粘合、萌发及花粉管在柱头表面和花柱中的生长情况。试验结果表明:甘蓝×白菜杂交的主要障碍在于白菜萌发的花粉管难以伸进柱头,并与柱头表面发生严重的胼胝质反应,使花粉管扭曲,尖端膨大,难以生长。
The cross was made between cabbage as female parent and Chinese cab-. bage. Ovaries of different periods after pollination were collected and stained with aniline blue. The degree of pollen-stigma binding, germination of pollen grains and growth of pollen tubes in stigma surface and style were observed under fluroscent microscope. The results showed that the main barrier to cross was that pollen tubes failed to penetrate stigma whose surface produced serious callose reaction, giving rise to the distortion of pollen tubes, swelling of their tops and failure to growth.
出处
《西北农业大学学报》
CSCD
1992年第3期1-6,共6页
Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)
基金
国家自然科学基金
关键词
甘蓝
白菜
远缘杂交
花粉
柱头
cabbage, Chinese cabbage, distant hybridzation, interspecific incompatibility, pollen-stigma interaction
