摘要
目的 建立胎鼠海马细胞缺氧模型 ,探讨硫酸镁对未成熟神经元细胞的保护作用。方法 体外原代培养孕 18d胎鼠海马神经元细胞 ,分别使用硫酸镁终浓度为 0、1、2、4mmol/L ,观察不同浓度硫酸镁对神经元的保护作用。结果 硫酸镁浓度为 1mmol/L和 2mmol/L实验组有神经元明显保护作用 ,在培养 2、4、8h后死亡率明显降低 ,在 2mmol/L谷氨酸损伤下c fos表达明显减少 ,以 1mmol/L浓度硫酸镁的实验组更明显。结论 硫酸镁在体外培养神经元细胞受到谷氨酸损伤时有保护作用。硫酸镁对体外培养海马神经元细胞受谷氨酸损伤时的保护作用在 2～ 4h较明显 ,在作用 4～
Objective It has become more important to protect brain function in operation and post-operation period with the developmen of surgery therapy for congenital heart surgery.Methods The primary cultured neuron cell of l8-day-pregnan embryoid Sprague-Dawley rat's hippocampus has been selected as the experimental material.The experimental concentration of magnesium sulfate will be 0 mmol/L,1 mmol/L,2 mmol/L,4 mmol/L;0 mmol/L is for control group.Selecting c-fos protein as target for primary antibody.Results After immunohistochemistry procedure,we found the mortality of neuronal cultures with magnesium sulfate in different concentration was 89.47 %,49.35 %,52.56 %,90.67 %(P<0.01).Conclusions The results reiterate the neuroprotection of magnesium sulfate.The results also show the effective time of magnesium sulfate is 2 to 4 hours. After 4 to 8 hours,the neuroprotection has become faint.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2004年第1期49-51,共3页
Journal of Applied Clinical Pediatrics
