摘要
目的 :探讨腹腔注射兴奋性氨基酸对大鼠海马神经元及Bax/bcl 2表达的影响。方法 :将大鼠随机分成两组 ,实验组给予腹腔注射卡因酸 ,对照组给予腹腔注射等体积磷酸盐缓冲液 (PBS) ;3天后以尼氏染色海马神经元 ,TUNEL法检测海马神经元凋亡 ;Bax/bcl 2抗体标记神经元Bax/bcl 2蛋白 ,SABC法检测。结果 :实验组大鼠海马神经元带变狭 ,TUNEL染色阳性对照片可见大量阳性细胞 ,阴性对照片未见阳性细胞 ;实验组Bax为强阳性 ,Bcl 2为阳性 ;PBS生理盐水对照组Bax为弱阳性(+) ,Bcl 2均为强阳性 ( )。结论 :腹腔注射卡因酸可以引起大鼠海马神经元凋亡 ,Bax表达上调 ,Bax/bcl 2平衡改变可能与神经元凋亡有关。
Objective:To study the influence of kainate on the hippocampal neuron of rats and the expression of Bax/bcl 2.Methods:The rats were divided into two groups randomly.The experimental group was given intraperitoneal administration of kainate,and the control group was given phosphate buffered saline solution(PBS).After 3 days,the loss of neuron in the hippocampus of rats was detected by Nissl staining.The neuronic apoptosis in the hippocampus was examined with terminal deoxynucleotidyl transferase mediated d UTP biotin nick end labeling(TUNEL);related gene Bax and Bcl 2 expression were marked by SABC method.Results:Kainate could induce neuron apoptosis and loss,up regulation of bax and down regulation of bcl 2 in the hippocampus of rats by intraperitoneal administration.Conclusions:The hippocampal neuron of rats can be impaired by intraperitoneal administration of kainate.Elevated expression of Bax/bcl 2 of neuron might be involved in the pathophysiologic course of apoptosis in neurons.
出处
《蚌埠医学院学报》
CAS
2004年第1期9-11,共3页
Journal of Bengbu Medical College
