摘要
目的 明确类固醇治疗瘢痕的具体作用机理。 方法 用细胞培养、免疫组织化学及分子生物学技术对 6例瘢痕疙瘩 ,6例增生性瘢痕患者及 6例正常人皮肤的成纤维细胞在激素作用下的细胞凋亡进行了研究 ;同时对 6例激素局部注射后的在体增生性瘢痕的成纤维细胞的增殖、生物合成及细胞凋亡进行了研究。 结果 (1)激素可以诱导体外培养的不同成纤维细胞的凋亡 ,同时伴有Bax/Bcl 2蛋白比率的升高。 (2 )局部注射激素可以通过抑制PDGF基因表达而抑制瘢痕成纤维细胞的在体增殖。 (3)局部注射激素可以通过抑制转录而抑制前胶原基因表达从而抑制在体瘢痕成纤维细胞的I、III型胶原合成。 (4)激素局部注射可引起瘢痕c myc和p5 3基因表达增高从而诱导在体瘢痕的细胞凋亡。 结论 激素治疗瘢痕的疗效是通过抑制增殖及生物合成促进细胞凋亡而实现的。
Objective[WT5”BZ] To investigate the mechanism of steroid in treatment of abnormal scars. [WT5”HZ]Methods[WT5”BZ] Apoptosis of different fibroblasts from 6 samples with keloid, 6 samples with hypertrophic scar, and 6 samples of normal skin was observed under the condition of the media containing steroid in vitro. Proliferation, biosynthesis and apoptosis of fibroblasts of 6 samples of hypertrophic scars treated with intralesional injection of steroid were studied in vivo. [WT5”HZ]Results[WT5”BZ] Steroid could induce apoptosis of different fibroblasts in vitro in correspondence with increasing radioes of Bax/Bcl 2 proteins. Intralesional injection of steroid could inhibit proliferation of fibroblasts from hypertrophic scars by inhibiting PDGF BB gene expression in vivo. Intralesional injection of steroid could inhibit procollagen gene expression to prohibit type I and III protein syntheses of fibroblasts from hypertrophic scars in vivo by inhibiting gene transcription. Intralesional injection of steroid could increase c myc and p53 gene expression of hypertrophc scars in vivo, which induced apoptosis of cells. [WT5”HZ]Conclusion[WT5”BZ] The effects of steroid on abnormal scars were achieved by inhibiting proliferation and biosyntheses of fibroblasts and promoting apoptosis of fibroblasts. [WT5”HZ]
出处
《中华外科杂志》
CAS
CSCD
北大核心
2000年第5期378-381,共4页
Chinese Journal of Surgery
