摘要
目的研究三七总皂苷(PNS)对小鼠肺纤维化肺组织细胞凋亡及凋亡调控因子Bax/Bcl-2的影响。方法将90只C57BL/6雄性小鼠分为假手术组、模型组和PNS组,每组30只。除假手术组外其余各组小鼠气管内一次性滴入盐酸博莱霉素(BLM),假手术组小鼠气管内一次性滴入等体积的生理盐水。各组动物于7、14、28d随机处死10只,取肺组织,检测肺组织细胞凋亡及Bax/Bcl-2的蛋白及基因表达水平。结果与相同时间点模型组相比,肺组织气道上皮细胞的凋亡明显减轻(P<0.01),Bax蛋白、Bcl-2蛋白、Bax mRNA及Bcl-2mRNA的表达明显降低(P<0.01或P<0.05)。结论肺纤维化时肺实质细胞凋亡增多,PNS通过调节Bax/Bcl-2的表达来抑制肺组织气道上皮的凋亡可能是其防治肺纤维化的作用机制之一。
Objective To investigate the effects of PNS on the cell apoptosis and apoptotic regulators Bax/Bcl-2 in mice pulmonary fibrosis.Methods 90 C57BL/6 male mice were divided into sham operation group,model group and PNS group（n=30）.In addition to the sham operation group,the remaining mice were conducted with a single intratracheal instillation of bleomycin hydrochloride;the mice in the sham operation group were conducted with a single intratracheal instillation with saline.10 mice were randomly chose and killed each time at 7 d,14 d and 28 d;extract the lung tissues to measure the apoptosis,Bax/Bcl-2 and gene expression.Results Compared with model group,apoptosis in the lungs of mice in model group were significantly higher than that in sham operation group（P0.01）;compared with model group,the Bax/Bcl-2 protein,expression of Bax mRNA and Bcl-2 mRNA in the PNS group were significantly lower（P0.01 or P0.05）.Conclusion Cell apoptosis would increase in pulmonary parenchymal,and one of the working mechanism for PNS to prevent and treat pulmonary fibrosis would be to inhibit cell apoptosis by regulating the expression of Bax/Bcl-2.
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第10期1125-1127,共3页
Chongqing medicine
基金
河北省科学技术研究与发展计划资助项目(11276432)
河北大学博士基金资助项目(2011)
