摘要
以MS为基本培养基 ,附加不同浓度的 6 BA、KT、NAA和IBA诱导洋桔梗叶片外植体的再生植株。结果表明 :MS +6 BA 0 .5～ 1 .0mg/L(单位下同 ) +NAA 0 .2和MS +6 BA 0 .5～ 1 .0 +IBA 0 .2培养基都能诱导外植体产生愈伤组织 ,但 6 BA的浓度必须小于 1 .0mg/L ,否则会导致组织的严重玻璃化 ;MS +KT 1 .0～2 .0 +NAA 0 .2或MS +KT 1 .0～ 2 .0 +IBA 0 .2培养基也能诱导外植体产生愈伤组织 ,愈伤组织出现的时间较早且质地较好 ,适合分化。继代培养时 ,MS培养基中仅加 6 BA 0 .5mg/L或KT 0 .5mg/L ,即能获得较高的分化率。生根培养研究中 ,培养液为 1 /2MS+5 0g/L糖 +IBA 2mg/L的前处理 ,生根效果较好 ,生根率接近基质生根培养的生根率。
The leaves explants from Eustoma sp. were cultured on MS medium supplemented with 6-BA,KT,NAA and IBA of different densities to induce regeneration plants. The results indicated that the callus can be induced on all medium of MS+6-BA 0.5～1.0 mg/L(Unit is the same as follows)+NAA 0.2 and MS+6-BA 0.5～1.0+IBA 0.2,but the density of 6-BA can not exceed 1.0 mg/L,otherwise,it will cause serious vitrification;At the same time,on these medium of MS+KT 1.0～2.0+NAA 0.2 or MS+KT 1.0～2.0+IBA 0.2,callus can also be induced early and its texture is better for differentiation. When subculturing,MS medium only supplemented with 6-BA 0.5 or KT 0.5 can obtain higher frequency of differentiation. When studying root regeneration culture,the explants that has been pretreated with culture liquid of 1/2 MS+50 g/L sucrose+IBA 2 mg/L had better root regeneration and root regeneration frequency was close to medium culture’s.
出处
《广西植物》
CAS
CSCD
北大核心
2004年第1期40-42,共3页
Guihaia
基金
四川省教育厅青年基金项目
