摘要
目的 :研究肠缺血再灌注 (I R)损伤后腹腔注射小剂量酵母多糖对全身炎症反应和远隔器官的影响。方法 :将雄性 Wistar大鼠随机分为 3组。二次打击组 (M组 )先阻断肠系膜上动脉血流 6 0 m in,于恢复肠道血流 12 h腹腔注射小剂量酵母多糖 (12 5 m g/ kg) ;对照组分别实施单纯肠缺血再灌注损伤 (I R)或腹腔注射酵母多糖 (Z)。观察 3组动物炎症介质、脏器功能及死亡率的变化。结果 :3组动物均出现全身炎症反应综合征 ,M组动物血肿瘤坏死因子、内毒素含量以及肺和小肠髓过氧化物酶活性均显著高于 I R组和 Z组。二次打击组 72 h多器官功能障碍综合征 (MODS)发病率和死亡率分别为 4 8%和 6 8% ,显著高于两个单次打击组。结论 :小剂量酵母多糖能诱发肠缺血再灌注后全身炎症反应和 MODS。
Objective:To investigate the effects of a small dose of intraperitoneal injection of zymosan following gut ischemiareperfusion injury on systemic inflammatory response and distant organ function. Methods: Wistar rats were randomly divided into three groups:gut ischemiareperfusion injury(IR) alone,intraperitoneal injection of zymosan(Z) alone,and gut ischemiareperfusion followed by intraperitoneal injection of zymosan (M).Gut (IR) was produced by occluding superior mesenteric artery for 60 minutes followed by gut reperfusion.A small dose(125 mg/kg) of zymosan was given intraperitoneally at 12 hours after recovery of gut blood flow.Results:Systemic inflammatory responses were found in all rats of three groups.The plasma level of tumor necrosis factorα and activities of myeloperoxidase in lungs and intestines were significantly higher in animals in M group compared with those in IR and Z groups.The incidence of multiple organ dysfunction syndrome(MODS) and mortality rate at 72 hours postinjury in twohits group were significantly higher than those in IR and Z group.Conclusion:A small dose of intraperitoneal injection of zymosan could induce systemic inflammatory response and multiple organ dysfunction more readily when there is a precedent gut ischemiareperfusion injury.
出处
《中国危重病急救医学》
CAS
CSCD
2003年第1期11-14,共4页
Chinese Critical Care Medicine
基金
军队医药科研"十五"指令性课题资助项目 (0 1L0 81)
