摘要
利用RAPD分子标记技术对83份狼尾草属(Pennisetum)牧草资源(其中7株为“23A”不育系中的可育株)进行遗传多态性聚类分析。结果表明:①共筛选200个随机引物,其中10个引物对所有供试材料都能扩增出清晰的多态性条带,且重复性好;②83份材料可分为5类,其中7株23A不育系里的可育株与23A单独聚成一类,初步确定其为不育系23A的育性逆转株;③引物S1038(TCGCGGAACC)能扩增出“杂交狼尾草”的特异指纹图谱标记,为牧草品种杂交狼尾草的保护提供了分子鉴定基础。
76 forage resources in Pennisetum and 7 fertile plants from male sterile line '23A' were analyzed by RAPD. The results showd-.①All materials appeared clear polymorphic band by using 10 primers selected from 200 primers. ②83 resources were divided into 5 groups. Since cluster Ⅲ only include 7 fertile plants from male sterile line 23A and sterile line 23A. It was basically confirmed 7 fertile plants from male sterile line 23A come from fertility reversion. ③A DNA fingerprinting amplified with S1038 ( TCGGGGAAGG) was constructed and that might be used in ' Hybrid Napier grass' identification.
出处
《江苏农业学报》
CSCD
2003年第2期109-113,共5页
Jiangsu Journal of Agricultural Sciences
基金
江苏省应用基础研究项目(BJ2000042)
