摘要
运用ISSR标记对采自云南的25份野生种质和4份驯化新品系中型狼尾草材料进行遗传多样性分析.结果显示：（1）50条ISSR引物中共筛选出10条能扩增出清晰条带且多态性明显的引物,29份材料DNA共获得72个扩增位点,其中多态性位点62个,多态性比率为87.4％,平均每条引物扩增位点为7.2个；平均观察等位基因数（Na）、有效等位基因数（Ne）、Shannon多样性信息指数（D和Nei＇s基因多样性指数（H）分别为1.861 1、1.742 8、0.561 0和0.395 9；种质材料间的遗传相似性系数变幅为0.236～0.903,表现出丰富的遗传多样性.（2）利用UP-GMA聚类分析,以遗传相似系数0.51为界,29份材料划分为4大类,但Mantel检测表明29份种质材料的遗传聚类和地理距离之间不存在显著的正相关关系（r=0.437 0,P=0.204 6）.研究结果首次从分子水平揭示了中型狼尾草的遗传多样性和变异水平,为合理地引种、驯化、保护和利用中型狼尾草野生资源提供了重要的参考依据和数据支持.
The genetic diversity of the 29 accessions of Pennisetum longissimurn var. interrnedium from Gan- su and Yunnan were analyzed by ISSR makers. The results showed that： （1）10 primers were selected from 50 primers which had clear,stable polymorphic bands. A total of 72 loci were obtained,including 62 poly- morphic loci. On average,amplification site of each primer was 7.2 and the percentage of polymorphic loci （PPL） was 87.4%. The mean observed number of alleles （Na） ,effective number of alleles （Ne） ,Shannon information index （1） and Nei;s genetic index （H） were 1. 861 1,1. 742 8,0. 561 0 and 0. 395 9, respective- ly. The genetic similarity coefficient （GS） ranged from 0. 236 to 0. 903,which shows rich genetic diversity among the materials of P. longissimum var. intermedium. （2）Cluster analysis with UPGMA method showed that the 29 tested accessions were divided into 4 groups at the level GS O. 51 ,but there was no cor- relation （r----0. 437 0, P ~ 0. 204 6） between genetic and geographic distance among the germplasms stud- ied. The results of this research were the first time to reveal genetic diversity and variation of P. longissi- mum var. intermedium at the molecular level, which provided theoretical and data evidence for the introduc- tion, domestication, conservation and exploitation of wild P. longissimum var. intermedium germplasm resources.
出处
《西北植物学报》
CAS
CSCD
北大核心
2014年第2期256-264,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(31201841)
甘肃省农业科技创新项目(GNCX-2013-58)
中央级公益性科研院所基本科研业务费专项资金(1610322014015
1610322013011)
兰州市科技计划项目(2011-1-167)
关键词
中型狼尾草
ISSR标记
种质资源
遗传多样性
Pennisetum longissimum var. intermedium ~ ISSR markers ~ germplasm resources ~ genetic diversity
