摘要
以玉米大斑病菌 (Exserohilumturcium)菌株 9948N为供试菌株 ,进行了原生质体制备条件的研究。主要探讨了酶、渗透压稳定剂、温度、时间等条件对原生质体产出率的影响。结果表明玉米大斑病菌原生质体制备的适宜条件是 :2 8℃下以 1 0mg mL纤维素酶 +1 0mg mL蜗牛酶的混合酶处理经过研磨的菌体 ,酶解 72h ,以 0 .8mol
The strain 9948N of Exserohilum turcicum was used to prepare the protoplasts in this paper. The conditions of isolation of protoplasts was discussed. The yield of protoplasts was significantly correlated with enzyme, osmot ic stabilizer, temperature and treatment time. The optimal condition shown in t his paper was that the ground mycelium of E. turcicum was trea ted by mixed enzymes (10 mg/mL Cellulase and 10 mg/mL Snailase) for 72 hours, an d the mixed enzymes are dissolved with 0.8 mol /L mannitol osmotic stabilizer .
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2003年第3期79-81,115,共4页
Journal of Hebei Agricultural University
基金
国家自然基金项目资助 (39970 4 77)
