摘要
为了利用原生质体融合技术获得同时具备固氮、解磷、抑病等活性的多功能菌株,本文对褐球固氮菌A41和荧光假单胞菌P32原生质体的制备条件进行了研究。通过对菌龄、酶解温度、酶浓度、酶解时间等影响因素的正交实验确定了褐球固氮菌A41和假单胞菌P32原生质体制备的最佳条件。实验结果表明,固氮菌A41最佳制备条件为菌龄20h、酶解温度37℃、酶浓度3mg/mL、酶解时间45min;假单胞菌P32最佳制备条件为菌龄20h、酶解温度37℃、酶浓度2mg/mL、酶解时间30min。
To got the multifunction trains that have Nitrogon fixation activities, Phosphat-solubilizing and disease suppressive simultaneously by protoplast fusion technology, conditions about protoplast optimization preparation of Azotobacter chrococcum 41 （A41） and Psedomonas fluorescens 32 （P32） were studied by orthogonal experiments, including the cell incubation time, the concentration of lysozyme, lysing time, etc. The results showed that the optimum preparation conditions for A41 protoplast was that the cell incubation time 20h, temperature 37℃, lysozyme 3mg/mL, lysing time 45min, respectively; and for P32, 20h, 37℃, 2mg/mL and 30min, respectively.
出处
《山东科学》
CAS
2006年第6期15-18,共4页
Shandong Science
基金
山东省科技攻关重点项目(项目编号:2005GG3209057)
