摘要
目的 对 4 6例SARS患者 2 0 6份粪便及漱口液标本 ,检测SARSCoV ,探讨SARS患者恢复期排毒的情况。方法 对住院的 4 6例临床确诊SARS病人 ,留取粪便及同期漱口液各 10 3份 ,提取RNA后利用 14对SARSCoV特异性引物 ,对每份标本同时进行 7次套式RT PCR扩增 ,凡 1次RT PCR结果阳性 ,该标本即判定为阳性。结果 10 3份粪便标本中 ,6 3份 (6 1.2 % )SARSCoV阴性 ,4 0份(38.8% )阳性 ,阳性者的病程为 30 .75d± 11.2 7d(12d～ 6 4d)。 10 3份漱口液标本中 ,81份 (78.6 % )阴性 ,2 2份 (2 1.4 % )阳性 ,阳性者的病程为 2 9.82d± 12 .4 6d(12d～ 6 4d)。病程最长 6 4d时粪便及漱口液中依然发现SARSCoV病原学依据。患者同期粪便及漱口液RT PCR检测结果一致的有 6 1份(5 9.2 % ) ,不一致的有 4 2份 (40 .8% )。结论 SARS患者恢复期排毒可长达 6 4d ,对SARS患者恢复期的消毒隔离应该得到足够的重视。SARS患者粪便SARSCoVRNA阳性 ,而同期漱口液SARSCoVRNA阴性 ,提示SARSCoV可以直接经消化道传播。
Objective To test the presence of SARS virus in convalescent stool sample and gargle by RT-PCR. Methods 206 stool and gargle samples from 46 SARS patients were simultaneously collected on May 16 th , 20 th , and 23rd, 2003. For each sample, RNA was extracted and 7 nest RT-PCR using 14-pair different SARS CoV special primers were carried out. Results Among 103 stool samples, 63 cases (61.2%) were SARS CoV nest RT-PCR negative. 40 cases (38.8%) were positive. The duration of positive cases was 30.75?d±11.27?d(12?d-64?d). In gargle samples,the negative rate was 78.6% (81/103) and positive rate was 21.4% (22/103). The duration of positive cases was 29.82?d±12.46?d (12?d-64?d). The longest duration of stool and gargle shedding in this study was 64 days. The coincident rate between stool and gargle samples was 59.2% (61/103). The discrepancy rate was 40.8% (42/103). Conclusion The longest stool and gargle shedding of SARS patient was 64 days after the first symptom of SARS in this study. So it is very important to execute exact sterilization of patients′ secretions and isolation of SARS convalescent patients. The possible source of stool shedding virus was alimentary SARS CoV infection foci rather than sputum swallowed.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2003年第12期930-932,共3页
Chinese Journal of Microbiology and Immunology
