摘要
本文将苏云金芽孢杆菌以色列亚种(Bacillus thuringiensis subsp.israelensis)130kDa杀蚊蛋白基因亚克隆到 pNQ122载体上,通过枯草芽孢杆菌(Bacillus subtilis)原生质体转化,得到 Km′Cm^(?)的正反向克隆子(pFZ1和 pFZ2)。Western-blotting 免疫杂交证明130kDa 杀蚊蛋白基因在枯草芽孢杆菌中表达了具有免疫活性的130kDa 杀蚊蛋白。所表达的杀蚊蛋白在实验中具有杀蚊活性。
Two recombinant plasmid pFZ1 and pFZ2 containing Bti 130kDa mosquitocidal proteingene in opposite insertion orientation were constructed.The expression of 130kDa mosquitoci-dal protein of Bti in Bacillus subtilis was comfirmed by western blotting.The mosquitolarv-cidal activity against the larvae of Aedes albopictus was shown by the bioassay.
出处
《微生物学报》
CAS
CSCD
北大核心
1992年第6期394-399,共6页
Acta Microbiologica Sinica
关键词
苏云金杆菌
基因表达
以色列亚种
Bacillus thuringtensis subsp.israelensis
Gene encoding for 130kDa protrein
Gene expression in Bacillus subtilis
