摘要
目的 研究有感染性的人巨细胞病毒 (humancytomegalovirus ,HCMV)和紫外线 (ultravioletlight,UV)灭活丧失感染性的病毒 (CMV UV)感染人胚肺成纤维细胞 (humanembryoniclungfibroblasts ,HLF)后白细胞介素 6(inter leukin 6,IL 6)mRNA的变化 ,并初步探讨中药金叶败毒制剂治疗HCMV感染的分子机制。方法 采用细胞培养与逆转录聚合酶链反应 (RT PCR)技术 ,研究IL 6在转录水平的表达。结果 UV灭活丧失感染性的HCMV与有感染性的HCMV均可上调IL 6mRNA的表达 ,中药金叶败毒制剂与更昔洛韦 (ganciclovir,GCV)都可抑制HCMV诱导的IL 6表达的上调。结论 IL 6mRNA的上调不需要有感染能力的HCMV ,中药金叶败毒制剂可抑制HCMV诱导的IL
Objective To study the expression of interleukin-6 mRNA in human embryonic lung fibroblas ts infected with human cytomegalovirus (HCMV) or ultraviolet light-inactivated human cytomegalovirus (AD169) and to determine the molecular mechanism of Jinyeb aidu in treating HCMV infection. Methods The expression of IL-6 at transcriptional level was det ected in human embryonic lung fibroblasts by means of cell culture and RT-PCR. Results Both ultraviolet light-inactivated HCMV and HCMV coul d induce up-regulation of IL-6. Both GCV and Jinyebaidu could suppress the up -regulation of IL-6 mRNA level. Conclusion Up-regulation of IL-6 occurs independently from pr oductive infection. Jinyebaidu could inhibit the up-regulation of IL-6 gene ex pression induced by HCMV.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2003年第6期637-640,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金资助项目 (No .3 0 2 71673 )
