摘要
目的探讨中药金叶败毒制剂(简称中药)对人巨细胞病毒(HCMV)感染人胚肺成纤维细胞(HEL)细胞病变及细胞周期的影响。方法用HCMVAD169感染人胚肺成纤维细胞,分别用金叶败毒制剂和更昔洛韦(简称GCV)干预后,用倒置相差显微镜观察不同感染时间细胞病变,并采用流式细胞技术检测HCMV感染及药物干预后宿主细胞周期。结果金叶败毒制剂能明显抑制HCMV感染HEL细胞病变。中药干预组在病毒感染48h后,HEL细胞处于G2/M期的细胞明显增多,在HCMV感染72h后,药物干预组HEL细胞均表现为S期细胞明显减少,G2/M期和G0/G1期细胞明显增多。结论金叶败毒制剂可能通过延缓HCMV感染细胞病变,促进细胞周期进程而发挥抗病毒作用。
TitleEffect of Jinye Baidu Preparation on Cell Pathological Change and Cell Cycle of Human Embryonic Lung Fibroblast Cells Infected by Human Cytomegalovirus AuthorYUAN Hui1,WEN Liang-zhen2（1.Medical College,Jianghan University,Wuhan 430056,China;2.Department of Gynecology and Obstetrics,Tongji Hospital,Tongji Medical College,Huazhong University of Sciences and Technology,Wuhan 430030,China） Objective To study the effect of Jinye Baidu Preparation（JBP）,a Chinese medicinal preparation,on the cell pathological change and cell cycle of human embryonic lung fibroblast cells（HEL）infected by human cytomegalovirus（HCMV）.MethodsThe HEL cells were infected by HCMV AD169,the pathological change of the infected cells during different infected time was observed by inverted contrast microscope before and after intervention of JBP and GCV.The cell cycle was detected in the HEL and the infected cells before and after intervention of medicine by the Flow Cytometer.ResultsJBP showed obviously inhibitory action on cell pathological change in the HCMV infected cells.The infected cells in G2/M phase were obvious increased 48 hours after HCMV infection after intervention of JBP.The infected cells in S phase were obviously reduced,and in G2/M phase and in G0/G1 phase they were obviously increased 72 h after HCMV infection after intervention of JBP.ConclusionJBP can delay cell pathological change of the HCMV infected cells,advance the cell cycle process so as to achieve the anti-virus action. KeywordsJinye Baidu Preparation;Human cytomegalovirus;Cell pathological change;Cell cycle
出处
《时珍国医国药》
CAS
CSCD
北大核心
2010年第9期2137-2139,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.30271673)
湖北省自然科学基金(No.2007ABA267)
湖北省教育厅资助项目(No.B200734001)
关键词
金叶败毒制剂
人巨细胞病毒
细胞病变
细胞周期
Jinye Baidu Preparation
Human cytomegalovirus
Cell pathological change
Cell cycle
