摘要
目的 建立一种检测血浆活化部分凝血酶时间 (APTT)的压电石英晶体传感器。方法 采用AT切向、基频 1 0MHz的银膜石英晶体作为压电元件 ;观察血浆凝集过程中晶体振荡频率的变化 ,确定血浆凝集反应的起点和终点。结果 (1 )血浆标本加入反应体系后 ,晶体振荡频率呈阶梯状快速下降 ,接着频率瞬时上升 ,然后再缓慢降低达到平稳 ,频率阶梯下降的起点为血浆凝集反应的起点 ,频率从快速上升变为下降的转折点为血浆凝集的终点 ,计算两点之间的时间即为血浆活化部分凝血酶时间。(2 )该方法检测结果与STAGO磁珠检测法的相关性好 (γ =0 .972 ) ,批内CV =2 .5 4 % ,批间CV =7.1 4 %。结论 压电石英晶体传感器检测血浆活化部分凝血酶时间 ,血浆凝集反应起点和终点确定方便、可靠。且操作简单、快速、成本低廉 ,检测结果准确 ,是一种敏感性高、重复性好的血浆活化部分凝血酶时间检测新方法 ,可用于临床常规检测。
Objective To establish a piezoelectric quartz crystal sensor for determination of active partial thrombin time(APTT)of plasma.Methods The quartz crystal was 10MHz quartz AT cut with silver coated electrodes.The oscillating frequency changes were observed in the course of the coagulation reaction of plasma sample,the starting point and the end point of the coagulation reaction were showed by the frequency change tendency.Results After CaCl2 solution was added into the reaction system to start the plasma coagulation reaction,oscillation frequency of the crystal was decreased as a ladder,then rose abruptly and descended quickly to balance.The start of the ladder like frequency decrease was regarded as the start point of coagulation reaction,the turning point of the frequency from rise to decrease as the end point of the reaction.The time between the start point and the end was calculated as APPT.It was showed there was a significant correlation between the results from our method and STAGO method(γ=0.972),the variances within and between batches were 2.54% and 7.14% respectively.Conclusion Our method for determination of the thrombin time of plasma is simple,rapid,specific,accurate,cheap,and it might be a valuable method for detection of APTT in clinical laboratory.
出处
《重庆医学》
CAS
CSCD
2003年第12期1608-1610,共3页
Chongqing medicine
基金
国家 8 63计划重大专项 (2 0 0 2AARZ2 0 2 3)
国家科技部九五科学仪器攻关项目<科学仪器和实验条件研究与开发>(96 A2 3 0 4 0 4 )
国家自然科学基金资助项目 (30 2 70 338)
重庆市科委攻关指导课题
军队"九五"
"十五"课题 (98M 0 64
0 1MA1 80
关键词
压电
石英晶体
传感器
凝血酶时间
piezoelectricity
quartz crystal
sensor
active partial thrombin time (APTT)
