摘要
为了将压电免疫传感器应用于急性白血病的临床免疫分型 ,首次研制了一种以等离子体聚合膜和金纳米粒子自组装技术为基础的新的压电免疫传感器 ,用于吸附固定多种淋巴细胞表面标志分子的单克隆抗体 ,检测急性白血病人血样中的相应分化抗原。结果表明 :该方法的检出率与临床常用的荧光免疫法相接近 ,无显著性差异(χ2 =3.4 ,P >0 .0 5 )。结论 :本研究中的免疫传感检测法具有成本低、操作方便和能进行实时监测与定量检测等优点 ,将其应用于急性白血病的临床免疫分型是可行的。
The aim of this study was to apply a new type piezo-electric immunosensor in immunology classfication for acute leukemia. Based on the plasma- polymerized film and the nanogold particles self-assembly technology,a new type piezo-electric immunosensor was firstly developed to absorb and fix the monoclonal antibodies for detecting leukemia cell antigens in patient blood samples. The results showed that the positive rate of detection with this method was quite close to that with the fluoroimmunoassay which are commonly used in clinic work. There was no significant difference between the positive rates (χ 2=3.4, P>0.05). It is concluded that this piezo-electric immunosensor detection has many advantages,such as convenient operation,real time operation and control,qualitative detection,and less cost. This method can be used for acute leukemia immunology classification.
出处
《中国实验血液学杂志》
CAS
CSCD
2004年第4期508-512,共5页
Journal of Experimental Hematology
