摘要
目的 :了解优选技术对人精子染色体及超微结构的影响。 方法 :分别应用上游法、Percoll密度梯度离心法及双曲管优选法处理精液 ,观察优选前后精子染色体畸变率、精子性染色体比例及超微结构相对正常的精子百分率。 结果 :精子染色体畸变率、精子性染色体比例及超微结构相对正常的精子百分率在 3种优选方法优选前后均无显著改变 (P >0 .0 5 )。 结论 :3种优选方法不会增加精子超微结构的损害 。
Objective: To know the effect of different selection techniques on chromosome abnormality rate and the percentage of spermatozoa with relatively intact head ultrastructure in human sperm. Methods: The selection techniques of swim-up, Percoll density gradient centrifugation and doule-flection tube were used to observe sperm chromosome abnormality rate, sex chromosomal ratio and the percentage of spermatozoa with relatively intact head ultrastructure. Results: The sperm chromosome abnormality rate, sex chromosomal ratio and the percentage of spermatozoa with relatively intact head ultrastructure remained unchanged after the application of the three selection techniques(P> 0.05). Conclusion: The three selection techniques used in this study neither increase the damage to sperm ultrastructure nor exert effects on human chromosome.
出处
《中华男科学杂志》
CAS
CSCD
2004年第1期29-31,共3页
National Journal of Andrology
