摘要
目的 评价低温冷冻对人精子受精能力的影响。方法 用人精子与去透明带金黄地鼠卵异种体外授精技术 (穿卵实验 ) ,比较冷冻前后及不同冷冻方法间精子的穿卵率 ,评估低温冷冻对人精子受精能力的影响。结果 精子穿卵率在冷冻前为(36 .4 1± 8.75 ) % ,经速冻法冷冻后为 (2 4 .6 3± 7.75 ) % ,经缓冻法冷冻后为 (31 .2 0± 7.2 3) % ,任意两者间比较结果均有显著差异(P <0 .0 1 )。结论 低温冷冻使人精子的受精能力显著下降 ;降温速度越快 ,受精能力下降越显著。在冷冻前后进行精子穿卵实验 。
Objective To evaluate the effect of cryopreservation on the ability of fertilization of human sperm.Methods Using a method of in vitro fertilization of zona free hamster oocytes with human sperm(sperm penetration assay),by comparing the frequencies of human sperm to penetrate specially prepared hamster ova before and after freezing and different freezing methods,to evaluate the effect of cryopreservation on the ability of fertilization of human sperm.Results The results indicated significant differences between fresh (36.41±8.75)%,fast frozen (24.63±7.75)% and slow frozen sperm (31.20±7.23)% in frequencies of human sperm to penetrate ovum( P <0.01).Conclusion These studies indicate that the ability of fertilization of human sperm descends significantly after freezing.The more rapid to reduce the temperature,the more significant the ability of fertilization of human sperm descends during the sperm cryopreservation.The sperm penetration assay will provide important clinical value before and after freezing.
出处
《重庆医学》
CAS
CSCD
2004年第5期686-687,共2页
Chongqing medicine
基金
四川省计划生育委员会基金资助 (970 1 3)
关键词
精子
冷冻保存
穿卵实验
sperm
cryopreservation
sperm penetration assay
