摘要
目的探讨脊髓损伤 (SCI)后慢性中枢性疼痛 (CCP)与P物质的关系。方法选取SD大鼠 2 8只 ,分为正常组 (A组 )、假手术组 (B组 ) ,以及用WADE法复制出SCI后无CCP组 (C组 )和CCP组 (D组 )。取大鼠T13 和L2 脊髓节段 ,采用免疫荧光组织化学染色法结合激光共聚焦显微镜技术观察脊髓背角P物质 (SP)的变化。结果各组大鼠T13 和L2 节段脊髓背角SP含量比较为 :D组较C组减少 (P <0 .0 5 ) ,较A组和B组明显减少 (P <0 .0 1) ;C组较A组和B组减少 (P <0 .0 5 ) ;A组与B组无显著性差异。结论SCI后CCP大鼠脊髓背角SP可能对CCP有某种程度的抑制作用。
Objective To approach the neurobiochemical mechanism of chronic central pain (CCP) after spinal cord injury (SCI). Methods 28 SD rats were divided into four groups, the normal group (group A), the pseudosurgery group (group B), and groups with CCP (group C) and without CCP (group D) after L 1 spinal cord section injured with WADE method. T 13 and L 2segments of rats' spinal cord were took and concentration changes of substance P (SP) in the spinal dorsal horn between two sections were examined by immunofluorescence histochemistry staining combined with confocal laser scanning microscope. Results Concentration of SP in the group D was decreased significantly compared with groups C, A and B ( P<0.05-0.01), that of the group C was less than that of group A and B ( P<0.05). Conclusion The rat model established by WADE method is proper to study CCP after SCI. SP in dorsal horn of spinal cord may inhibit the CCP after SCI in some degrees.
出处
《中国康复理论与实践》
CSCD
2003年第12期719-721,共3页
Chinese Journal of Rehabilitation Theory and Practice
关键词
脊髓损伤
慢性中枢性疼痛
脊髓背角
P物质
免疫组织化学法
共聚焦激光扫描显微镜
spinal cord injury (SCI)
chronic central pain
spinal dorsal horn
substance P (SP)
immunohistochemical assay
confocal laser scanning microscope
