摘要
目的探讨P物质（P substance,SP）对脊髓星形胶质细胞活化和分泌炎性因子TNF-α、IL-1β的影响.方法 体外培养脊髓星形胶质细胞,施加SP刺激后分别应用RT-PCR和Western blot测定胶质原纤维酸性蛋白（glial fibrillary acidic protein,GFAP）mRNA和蛋白的表达,ELISA法检测肿瘤坏死因子-α（TNF-α）、白介素-1β（IL-1β）分泌的变化.结果10^-7mol/L SP的作用下,星形胶质细胞GFAP mRNA和蛋白的表达在0～72 h逐渐升高,与正常对照组相比差异显著（P＜0.01）;SP在10^-9～10^-6mol/L浓度范围内呈浓度依赖性促进星形胶质细胞分泌TNF-α、IL-1β（12 h）,与对照组有显著差异（P＜0.01,P＜0.05）,但IL-1β在SP 10^-6mol/L作用组下降,与对照组无明显差异（P＞0.05）.结论 SP刺激下脊髓星形胶质细胞明显活化,分泌炎性致痛因子TNF-α、IL-1β增多,表明周围慢性疼痛可能通过致痛递质SP介导下星形胶质细胞的活化引起脊髓中枢神经炎性痛.
Objective To explore the influence of SP on the activation and excretion of TNF-α, IL-1β of astrocytes of spinal cord. Methods After the astrocytes of spinal cord had been cultured in vitro and stimulated by 10^-9, 10^-8, 10^-7, 10^-6 mol/L SP for 12 h, or 10^-7 mol/L SP for 0, 24, 48, 72 h, the GFAP expression was investigated by RT-PCR and Western blotting, TNF-α and IL-1β by ELISA. The astrocytes cultured in serum-free medium only containing ITS served as control. Results SP （ 10^-7mol/L） promoted the expression of GFAP mRNA and protein in experimental astrocyes of spinal cord gradually on the time range of 0 - 72 h with marked difference with control （P 〈0.01 ）. SP caused a concentration-dependent （10^-9 -10^-6 mol/L, 12 h） increase of TNF-α, IL-1β excretion with marked difference between the experimental and control （P 〈0.01 ）, except for 10^-6 mol/L SP, which caused no obvious difference of IL-1β excretion. Conclusion Stimulated by SP, the astrocytes of spinal cord could be activated and excreted more proinflammatory cytokines such as TNF-α and IL-1β, which indicated that the chronic surrounding pain might result in the inflammation and pain of spinal cord with the transduction of transmitter SP.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第7期681-684,共4页
Journal of Third Military Medical University
