摘要
目的 检测低剂量辐射 (LDR)对小鼠腹腔巨噬细胞IL 12的影响 ,同时观察胸腺细胞CD2及腹腔巨噬细胞表面分子CD48的变化并分析其与IL 12变化的关系。方法 分别采用Northernblot和ELISA检测了 0 0 75GyX射线全身照射后IL 12转录水平、蛋白水平的变化 ;采用荧光免疫流式细胞术检测CD2、CD48蛋白表达的变化。结果 (1)IL 12的改变 :0 0 75GyX射线全身照射后 1h巨噬细胞中IL 12、p3 5及p40亚基mRNA水平均迅速升高 ,分别达到假照组的 13 1%和 192 %(P <0 0 5) ,而后开始回降直至照后 16～ 48h恢复正常 ;巨噬细胞分泌IL 12在照后 4～ 8h明显增高(P <0 0 5)。 (2 )CD2、CD48表达变化 :0 0 75GyX射线照射后 4h胸腺细胞CD2表达即开始升高 ,至8h达峰值 (P <0 0 5～ 0 0 1) ;巨噬细胞CD48表达在照射后 2h开始升高 ,至 48h仍明显高于假照水平 (P <0 0 5～ 0 0 1) ;4h量效结果显示 ,50 ,75,10 0和 2 0 0mGy均使CD2、CD48表达上调 (P <0 0 5)。结论 LDR可引起IL 12表达上调 ,CD2、CD48分子与IL 12的变化具有一致性 ,提示两者可能参与IL
Objective To observe the changes in the secretion of IL-12 and the expressions of CD2 and CD48 in mice after low dose irradiation an d to analyses the possible relationship between them. Methods North ern blot and ELISA were used to detect the mRNA and the protein level of IL-12 respectively.Flow cytometry(FCM) was adopted to detect the changes in CD2 and CD 48 expression. Results (1)Changes in IL-12 mRNA levels of both IL-12 p35 and p40 subunits increased and reached their peaks at 1 h(131% and 19 2% of sham-irrad respectively) after WBI with 0 075 Gy X-rays and IL-12 secr etion by macrophages was found to increase from 4 h to 8 h after exposure (P <0^05).(2)CD2,CD48 expression:FCM data showed CD2 expression in thymocytes i ncreased beginning from 4 h,reaching its peaks, at 8 h after irradiation,while the increase of CD48 expression in peritoneal macrophages was found from 2 h and maintained at high level up to 48 h (P<0^05～0 01).The results of dose -effect study at 4 h after irradiation illustrated up-regulated expression of CD2 and CD48 after 0^050,0^075,0^100,0^200 Gy irradiation (P<0^05). Conclusion LDR might up-regulate the expression of IL-12,and the consistence of CD2 and CD48 with IL-12 changes suggested that they might part icipate in the immunological regulation by increased secretion of IL-12.;
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2003年第6期402-405,共4页
Chinese Journal of Radiological Medicine and Protection
基金
国家自然科学基金资助项目(39570 1 88)
