摘要
本文采用3-氨基邻苯二甲酰肼增强化学发光(CL)的方法检测8例特发性肺纤维化(IPE)患者和10例正常对照的肺灌洗液(BAL)全细胞的CL。IPF患者BAL细胞的自发发光显著高于对照(P<0.01),而其刺激发光竟高达对照的11倍(P<0.001),说明IPF患者肺组织的炎症细胞释放大量的反应性氧中间物(ROI),后者可能是造成肺损害的原因。此外还对5例IPF患者接受激素治疗前后BAL细胞的CL进行了比较,3例接受激素后临床明显好转,其BAL细胞的CL较治疗前显著下降(P<0.05),2例治疗后无好转的患者其BAL的CL与治疗前相比无显著性变化(P>0.05)。本文以化学发光的方法研究和探讨了特发性肺纤维化的发病机制。
Idiopathic pulmonary fibrosis(IPF) is a chronic inflammatory lung disease charac-terized by injury of the parenchymal cells and connective tissue components of thealveolar wall and a progressive fibrosis of the alveolar structure. Reactive oxygenintermediates(ROI) released by inflammatory cells seem to play a major role in tissuedamage of IPF. Luminal-enhanced Chemiluminescence(CL)of toroal bronchoalveolar lavage(BAL)was evaluated in ten control subjects and 8 patients with IPF. In comparison withcontrol, the spontaneous CL of BAL in patients with IPF was significantly higherthan that of the control (P<0.01). With zymosan, the CL of BAL in patients withIPF was about 11-fold higher than that of normal subjects(26.08±7.28 versus 2.25±1.20.P<0.001). The results demonstrated that in IPF great amounts of ROI were releasedby inflammatory cells of BAL, which might be the major cause of the lung lesions. In 3 patients with idiopathic pulmonary fibrosis who received corticosteroid mono-therapy and showed clinically effective, CL values of BAL were greatly reduced eom-pared to those before therapy (P<0.05). In two patients who didnot respond to cor-ticosteroids, however differences between BAL-CL values before and after therapywere not statistically significant. This indicated that ROI released by inflammatorycells of BAL reduced markedly the tissue damage of the lung after effective corticos-teroid therapy.
出处
《天津医药》
CAS
1992年第10期597-599,共3页
Tianjin Medical Journal
关键词
肺纤维化
肺灌洗液
炎症细胞
BAL
inflammatory cells
chemiluminescence
zymosan
