摘要
从籽草中分离纯化的SOD比活为1855 U/mg,纯化倍数约为100倍,总活力回收率22.6％,经SDS-PAGE测定,纯化的SOD纯度达电泳纯,该酶对氰化物和过氧化氢敏感,可证明其为Cu/Zn-SOD。
Superoxide dismutase (SOD) (EC 1.15.1.1.) was purified to homogeneity, from Radix Lithospermi seeds (RLS). Lipid and other pigments were removed by super critical fluid extraction (SCF). Stepwise isolation and purification procedure consisted of ammonium sulphate precipitation, ion exchange chromatograpgy on diethyl ammo ethyl-52 and gel filtration using Sephadex G-100. Pure RLS Superoxide dismutase had a specific activity of about 1855 units per milligram protein and was purified 100-fold, with a yield of 22.6 % . The purifired product was confirmed as electrophoresis purity with SDS-PAGE. The preliminary sensitivity experiments showed that RLS SOD was cyanide-and hydrogen peroxide-sensitive, similar to those reported for CuZn-SODs.
出处
《药物生物技术》
CAS
CSCD
2003年第6期358-362,共5页
Pharmaceutical Biotechnology
