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木聚糖酶基因在大肠杆菌中的表达及表达蛋白的纯化 被引量：12

Expression of Xylanase B Gene of Thermotoga maritima in Escherichia coli
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摘要 热海栖热袍菌Thermotogamaritima是一个嗜极端高温的厌氧细菌,其产生的耐高温和热稳定性的木聚糖酶B具有很好的工业应用前景.利用PCR技术将嗜热产乙醇菌T.maritima编码高度热稳定性木聚糖酶的基因克隆至原核表达载体pET-20b,使之与组氨酸标签融合,并在大肠杆菌JM109(DE3)中得到了高效表达.最后采用金属Ni2+螯和层析柱对基因表达产物进行了纯化. Thermotoga maritima is one of the few hyperthermophilic bacteria described to date. The xylanases found in T. maritima has shown the extremely thermostablity and considerable potential in largescale production. The gene of xylanaseB from T. maritime ATCC 43589 was amplified by PCR, inserted into the plasmid pET20b, and fused with the 6His tag. The resultant recombinant plasmid pET20bXynB was transformed to E.coli JM109(DE3). A gene product band can be seen with SDSPAGE. The recombinant protein was purified by heating and immobilizing metal affinity chromatography.
作者 薛业敏 曹建平 毛忠贵 邵蔚蓝
机构地区 江南大学工业生物技术教育部重点实验室
出处 《无锡轻工大学学报（食品与生物技术）》 CSCD 北大核心 2003年第3期57-61,共5页 Journal of Wuxi University of Light Industry
关键词 木聚糖酶 基因表达 大肠杆菌 纯化 热海栖热袍菌 嗜热产乙醇菌T.maritima 基因克隆 xylanase B PCR amplification expression isolation
分类号 Q786 [生物学—分子生物学]
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参考文献12

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二级参考文献22

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共引文献37

同被引文献139

引证文献12

二级引证文献73

无锡轻工大学学报（食品与生物技术）
2003年 第3期

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