摘要
目的:了解合成的拟TGF-e样多肽P18对于成骨细胞株MC3T3-E1的增殖及分化的影响。方法:MTT法观察P18对于培养MC3T3-E1细胞株的增殖的影响,竞争性半定量RT-PCR了解其对Ⅰ型胶原表达的作用,并观察了其对于成骨细胞碱性磷酸酶活性的作用。结果:P18增加成骨细胞株MC3T3-E1的Ⅰ型胶原的表达,但对于碱性磷酸酶活性无明显影响,多肽对于MC3T3-E1细胞增殖的影响依培养条件不同而异。结论:P18对于成骨细胞的作用与TGF-e类似,由于其还具有HA结合活性,在HA骨修复材料的改进以及种植体涂层修饰方面有着广阔的应用前景。
To explore the effect of synthesis TGF-β like peptide P18 on the proliferation and differentiation
of osteoblastic cell lineMC3T3-E1. Methods: ATGF-β like peptide named P18 with two functional group was synthesized
by employing solid phase peptide synthesis and assayed by reverse HPLC and mass spectrum. The effect of P18 on the
proliferation of MC3T3-E1 cell was observed by the method of MTT. The competitive hemiquantitive RT-PCR was used to determine the expression of collagen type I . The alkaline phosphatase activity was also observed under the existence of peptide. Results: The effect of P18 on effect on the proliferation on osteoblastic cell depends on the condition of cell culture. It can obviously enhancing the expression of collagen type 1 gene. However, no obvious effect was observed for the ALP
activity under the existence of 20uM PI 8. Conclusion: The effect of PI 8 on osteoblastic cell is similar to that of TGF-β. It
may have extensive application prospect in the modification of implant coating layer and improvement of hydroxyapatite based bone repair materials, as its hydroxyapatite binding property is mentioned.
出处
《口腔颌面修复学杂志》
2000年第1期36-39,共4页
Chinese Journal of Prosthodontics
