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不同温度下成骨细胞增殖及其OPG/RANKL mRNA表达的影响 被引量：1

Mild heat shock’s effect on the proliferation and OPG/RANKL mRNA expression in Osteoblast
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摘要 目的:探讨不同温度下对小鼠成骨细胞MC3T3-E1的增殖以及OPG/RANKL表达水平的影响。方法:1.以小鼠成骨细胞MC3T3-E1为体外实验模型,MTT法检检测细胞的增殖情况。2.RT-PCR方法检测MC3T3-E1OPG/RANKL mRNA的表达水平。结果:设定对照组为37℃,高于对照组(38℃-39℃-40℃-41℃-42℃)分别作用于MC3T3-E1细胞1小时/天,连续1周,可刺激细胞增殖,OD值显著增加(P<0.05)。同时可增加OPG mRNA表达,降低RANKL mRNA表达,呈温度梯度依赖性。结论:热刺激促进MC3T3-E1细胞增殖,同时通过调节OPG/RANKL mRNA的表达,直接促进骨形成,抑制骨吸收。 Objective：To study the effect of mild heat shock on proliferation and the expression of osteoprotegerin （OPG） and receptor activator of NFK B ligand （RANKL） mRNA in MC3T3-E 1 osteoblasts of mice.Methods：The osteoblasts MC3T3-E 1 were cultured in vitro,the effects of different degrees of miled heat on osteoblast proliferation were assessed by MTT colorimetry;the expression OPG and RANKL mRNA in MC3T3-E1 was analyzed by semi-quantative RT-PCR.Results：Proliferation of MC3T3-E1 was promoted by mild heat shock （37℃-38℃-39℃-40℃-41℃-42℃ ）after 1 h/d x lw,Semi-quantative RT-PCR examination revealed：Mild heat shock promote OPG mRNA expression and inhibit the RANKL mRNA expression, in temperature gradient-dependent manner.Conclusion： Mild heat shock promotes proliferation in MC3T3-E1 osteoblasts of mice,and promotes OPG mRNA expression and inhibits RANKL mRNA expression,accordingly promotes bone formation,and inhibits bone resorption.
作者 甘璐 吴进 周勇 杨彤涛
机构地区 第四军医大学唐都医院骨科
出处 《现代生物医学进展》 CAS 2010年第5期847-850,共4页 Progress in Modern Biomedicine
基金 陕西省自然科学基金(2007C243)
关键词 RANKL OPG 成骨细胞 增殖 Mild Heat Shock Proliferation Osteoblast OPG RANKL
分类号 Q95-3 [生物学—动物学] R322.71 [医药卫生—人体解剖和组织胚胎学]
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参考文献19

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二级参考文献38

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共引文献11

同被引文献33

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现代生物医学进展
2010年 第5期

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