摘要
目的 :通过体外药敏试验 ,研究胰腺癌细胞对化疗药物的敏感性 ;筛选敏感的化疗药物 ,指导临床化疗方案的制定 ,并讨论胰腺癌化疗个体化方案的意义。方法 :用MTT比色法检测 40例原代培养的胰腺癌细胞对氨甲蝶呤、5 氟脲嘧啶、丝裂霉素、长春地辛、羟基喜树碱、依托泊甙、顺铂、表阿霉素、吡柔比星和健择等 1 0种药物的敏感性。结果 :以上 1 0种药物对 40例原代胰腺癌细胞生长抑制率分别为 2 8 6± 1 7 9%、41 3± 2 0 7%、45 4± 2 3 0 %、38 4± 2 4 6 %、37 3± 2 1 5 %、39 5± 2 0 7%、37 0± 1 9 0 %、33 7± 2 1 0 %、38 4± 1 8.3%和 42 8± 1 9.5 % ,共有 33例 (83 % )筛选到了抑制率 >60 %的药物。结论 :体外培养的胰腺癌细胞对 1 0种化疗药物的敏感性较差 ;不同的胰腺癌细胞对不同的药物敏感。
Purpose: To study the drug sensitivity of the primary pancreatic adenocarcinomas to 10 chemotherapeutic drugs. Methods: The drug sensitivity test of ten chemotherapeutic drugs to pancreatic cancer cells derived from 40 cases of patients with pancreatic cancer was analyzed by MTT colorimetric assay. Results: The average inhibition rate of ten chemotherapeutic drugs was:methotrexate 28.6%,5-fliorouracil 41.3%,mitocim-C 45.4%,leurocridtine 38.4%,etoposide 37.6%,cisplatin 39.5%,epirubicin 37.0%,hydroxycamptothecin 33.7%,pirarubicin 38.4% and gemcitabine 42.8%,respectivly. There was no significant difference among these drugs. Conclusion: Using drug screening system to guide clinical individualization chemotherapy may be a benificial approach to improve the result of chemotherapy of pancreatic cancer.
出处
《临床消化病杂志》
2003年第6期263-264,共2页
Chinese Journal of Clinical Gastroenterology
关键词
胰腺肿瘤
MTT比色
药敏试验
多药耐药
Pancreatic neoplasms MTT colorimetric assay Drug-sensitivity test Multiple drug resistance
