摘要
目的 建立青辛速释贴中靛蓝的含量测定方法。方法 采用索氏提取,HPLC法测定青辛速释贴中靛蓝的含量,色谱柱:Shim—pack VP-ODS柱(4.6×250mm),流动相:甲醇-乙腈-0.1 mol/L乙酸铵(60:4:36),流速:1.0 mL/min,柱温:30℃,进样量:20 μL,检测波长:280 nm。结果靛蓝在0.44～6.60 μg/mL范围内呈良好的线性关系(r=0.9991);平均回收率为103.6％,RSD=1.11％。结论 该方法准确、灵敏、重现性好,可用于青辛速释贴的质量控制。
Objective To establish a method for the determination of indigo content in Qingxin Sushitie by HPLC. Methods At 30℃, HPLC was performed to determine indigo on ODS column(4.6×250 mm), the mobile phase was composed of methanol-acetonitrile-0.1mol/L ammonium acetate(60: 4: 36), flow rate was 1.0 mL/min; injection volume was 20 μL and detection wavelength at 280 nm. Results The linearity of indigo was good within 0.44～6.60 μg/mL (r=0.9991), the average recovery was 103.6% and RSD=1.11%. Conclusion This method is simple, sensitive and accurate, and can be used for quality control of Qingxin Sushitie.
出处
《中药新药与临床药理》
CAS
CSCD
2003年第6期398-400,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省中医药管理局科研课题 (编号 099628)
关键词
青辛速释贴/化学
靛卡红/分析
高压液相色谱法
@ Qingxin Sushitie/chemistry
Indigo carmine/analysis
Chromatography, high pressure liquid
