摘要
目的：观察蒺藜总皂甙（ｇｒｏｓｓｓａｐｏｎｉｎｓｆｒｏｍ ＴｒｉｂｕｌｕｓｔｅｒｒｅｓｔｒｉｓＬ，ＧＳＴＴ） 对肿瘤坏死因子－ α（ＴＮＦ－ α） 、白细胞介素－１β（ＩＬ－１β） 诱导的培养新生牛脑微血管内皮细胞（ＣＭＥＣ） 粘附大鼠血单核细胞（ＭＮＣ） 及中性粒细胞（ＰＭＮ） 的影响。方法：用ＴＮＦ－α和ＩＬ－１β分别诱导传代的ＣＭＥＣ，计粘附ＭＮＣ及ＰＭＮ的细胞数，并计算粘附率。结果：ＣＭＥＣ 经ＴＮＦ－ α处理４ｈ 后，ＣＭＥＣ与ＭＮＣ及ＰＭＮ的粘附率，分别增加到３１．３±０．５％ 和３２．１±０．５％ （对照组分别为１２ ．５±０ ．２％ 和１３ ．８±０ ．４ ％） ，经ＩＬ－１β处理２ｈ 后，则分别为４４．５ ±１．０ ％ 和４４．８ ±０．７ ％ （ 对照组分别为１２ ．９ ±０．６ ％ 和１４．７ ±０．６％ ）。在ＴＮＦ－ α（ＩＬ－ １β） 处理前，用ＧＳＴＴ与ＣＭＥＣ共孵育，预处理２ｈ，则ＧＳＴＴ可剂量依赖性地抑制ＴＮＦ－ α和ＩＬ－ １β的作用。结论：ＧＳＴＴ可抑制ＣＭＥＣ 与ＰＭＮ及ＭＮＣ的粘附。
AIM To study the effect of gross saponins from Tribulus terrestris L (GSTT) on adhesion of monocytes(MNC) and polymorphonuclear leukocytes(PMN)to cultured bovine cerebral microvessel endothelial cells(CMEC) induced by TNF-α and IL-β.METHODS The adhesive cell number of MNC and PMN to CMEC treated by TNF-αand IL-β was counted,and the cell adhesion rate was calculated.RESULTS CMEC were induced by TNF-α for 4h,the adhesion rate of MNC and PMN to CMEC were increased to 31.3+0.5% and 32.1+0.5%,respectively(the control groups were 12.5±0.2% and 13.8±0.4%);CMEC were treated by IL-1β for 2h,the adhesion rates of MNC and PMN were 44.5±1.0% and 44.8±0.7%,respectively(the control group was 12.9±0.6% and 14.7±0.6%).CMEC was incubated with GSTT for 2h before treated by TNF-α(or IL-1β),then it showed that GSTT inhibited the effect of TNF-α and IL-1β in a concentration-dependent manner.CONCLUSION GSTT inhibited the adherence of MNC and PMN to CMEC.
出处
《解放军药学学报》
CAS
1999年第1期6-8,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
国家自然科学基金
关键词
蒺藜总皂甙
TNF-Α
IL-1Β
内皮细胞
抑制作用
CSTI
粘附率
Tribulus terrestris L, vascular endothelium, monocytes, polymorphonuclear leukocytes, tumor necrosis factor, Interleukin-1
