摘要
从290个土样中分离到1380株细菌,加上本所其他课题组提供的细菌共1870株,其中有707株能分解淀粉,经过复筛、纸层析鉴定有3株菌的淀粉酶酶解液中主要产物是麦芽四糖,进一步用β-淀粉酶水解为麦芽糖,用萄葡糖淀粉酶水解为萄葡糖,确证为麦芽四糖。其中最优菌株为537.1,其酶解产物中麦芽四糖占90%,而其他两株菌的酶解产物中除麦芽四糖外,还有较多的麦芽糖及麦芽三糖,因此选择了537.1作为形成麦芽四糖淀粉酶的优良菌株,经鉴定,该菌属于产碱菌(Alcaligenes sp.)。菌株537.1产酶的较好条件为:培养基中麦芽糖1.5%,蛋白胨0.5%,起始pH7—7.5,在27—28℃振荡培养48h。菌株537.1培养液可以酶解谷类、薯类和野生植物淀粉生成麦芽四糖。
The authors isolated 1380 bacteria strains from 290 soil samples collected in China and 490 strains were granted by other research teams in this institute. By screening 707 strains showed starch-hydrolyzing activity. By further screening and paper chromatographic test, three strains with maltotetraose as the main product were obtained. The maltotetraose was further confirmed by treatment with [3-amylase spliting to maltose and with glucoamylase to glucose. The most promising strain was 537.1, which produced maltotetraose about 90% (w/w) in the starch hydrolysate. While the other two strains produced more maltose and maltotriose besides maltotetraose.Strain 537.1 was tentatively identified as Alcaligenes sp.The optimum conditions for enzyme production were as follows, medium composition: 1.5% maltose; 0.5% peptone with initial pH of 7.0—7.51 cultured at 27—28℃ for 48h on rotary shaker.The culture filtrate of the strain 537.1 can hydrolyze strach and different kinds of cereal meal with a high yield of maltotetraose in the hydrolysate.
出处
《生物工程学报》
CAS
CSCD
北大核心
1992年第3期288-293,共6页
Chinese Journal of Biotechnology
基金
国家自然科学基金
中国科学院资助项目
关键词
产碱菌
麦芽四糖
淀粉酶
发酵
Maltotetraose-forming amylase
Alcaligenes sp.
glucosidase
