摘要
过去已通过电镜定量研究观察到吗啡能够促进备用根大鼠(切断一侧脊神经L_(1-3)、L_5、L_6背根,保留L_4背根)在脊髓Ⅱ板层重建了突触联系。本文在此基础上,进一步探讨了备用根大鼠L_4背根内神经纤维的数量变化以及吗啡对神经纤维数量或侧枝出芽的影响。结果显示:1.有髓纤维:对照组大鼠手术侧与非手术侧L_4背根内有髓纤维的数量的比较无统计学差别,吗啡组两侧L_4有髓纤维的数量也无明显差别。2.无髓纤维:与对照组非手术侧L_4背根内无髓纤维数目比较,吗啡组手术侧与非手术侧的无髓纤维数分别有所增加,但两者的增加幅度无统计学差异;与对照组大鼠手术侧L_4背根内无髓纤维的增幅比较也无统计学差异。上述结果表明此时吗啡对备用背根内无髓纤维的侧支出芽无明显的影响。而对非手术侧相应背根无髓纤维的侧支出芽有促进作用;除备用根大鼠非手术侧外,吗啡备用根大鼠手术侧与非手术侧,备用根手术侧L_4背根内增加的无髓纤维可能参与了Ⅱ板层,特别是手术侧Ⅱ板层的突触重建。
Our previous study has shown that morphine might promote the synaptic re-innervation in spinal lamina Ⅱ from spared dorsal root in the rat by quantitative EM study, the present work explored the effect of morphine on the nerve fiber number, in other words, on the nerve fiber collateral sprouting of the spared dorsal root in the rat by sectioning unilaterally L1-3, L5-6 dorsal roots, sparing L4. The results showed: 1. Myelinated fibers—On both sides of the morphine group and on the un-operated side of the control group, no significant differences were seen in the number of L4 dorsal root fibers, indicating that there was no collateral sprouting in them. 2. Unmyelinated fibers—On the un-operated side of morphine group, the number of L4 dorsal root fibers was significantly larger than that of the other three sides (operated side of morphine group and 2 sides of the control group), while no significant differences were found among the latter. This implies that morphine does not promote collateral sprouting of the unmyelinated fibers in the spared dorsal root, but does so on the un-operated side. The increase of the unmyelinated fibers on the three sides might take part in the synaptic re-innervation of lamina Ⅱ, especially on the operated side.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
1992年第1期53-56,共4页
Chinese Journal of Neuroanatomy
关键词
吗啡
备用背根
侧支出芽
morphine
spared dorsal root
collateral sprouting
rat
