摘要
目的 用CD3 4标记胃癌微血管内皮细胞 ,测定肿瘤微血管密度 (MVD) ,探讨其与胃癌各临床病理因素的关系。方法 采用免疫组织化学S P法检测 5 2例胃癌、10例癌旁组织和 16例正常胃黏膜组织CD3 4抗原的表达 ,对CD3 4单克隆抗体标记的血管计数MVD。结果 CD3 4染色胃癌微血管具有高度的特异性、敏感性及清晰的背景 ,易于计数。胃癌组织MVD明显高于正常胃黏膜组织(P <0 .0 1)和癌旁组织 (P <0 .0 5 ) ;MVD与肿瘤大小、浸润深度、是否发生远处器官转移、TNM分期明显相关 (P均 <0 .0 5 )。结论 CD3 4是良好的血管内皮细胞标记物 ;胃癌血管生成与肿瘤的生长、浸润、转移及疾病进展密切相关 ;MVD可作为判断胃癌生物学行为 ,预测胃癌发生发展的指标。
Objective The aim of this study is to investigate the relationship of MVD and pathological factors of gastric carcinoma by use of CD34 monocolon antibody labeling vascular endothelial cell of gastric carcinoma.Methods The expressions of CD34 antigen in 52 ceases of gastric carcinoma tissues,10 cases of paracarcinoma tissues and 16 cases of normal gastric mucosa tissues were examined by use of S-P immunohistochemistry.To calculate the number of microvessel labelled by anti-CD34 monoclonal antibody.Results Staining of CD34 was easy to identify because of its high specificity,sensitivity and clear background.MVD in gastric carcinoma tissues was significantly higher than in normal gastric mucosa tissues ( P<0.01) and in paracarcinoma tissues( P<0.05). MVD was related to the size of tumor,depth of invasion,distant organ metastasis and TNM stage( P<0.05). Conclusion CD34 antigen is a good vascular endothelial cell marker.Angiogenesis is related to growth,invasion,metastasis and development of gastric carcinoma.
出处
《河南肿瘤学杂志》
2003年第5期319-321,共3页
Henan Journal of Oncology
