摘要
目的 探讨肥厚性瘢痕的发生与成纤维细胞和血管内皮细胞凋亡的关系，以及有关调控因素的影响。 方法 应用原位末端标记和免疫组织化学染色等技术，对61例烧伤后整形患者肥厚性瘢痕和20例其他手术患者非肥厚性瘢痕进行了细胞凋亡以及ICE、Bcl-2表达的检测。 结果 肥厚性瘢痕增厚期、成熟期成纤维细胞以及血管内皮细胞凋亡阳性细胞指数分别为6.60±4.43和8.90±6.01，成熟期分别为25.60±5.70和26.60±6.02，两期同种细胞间相比，凋亡阳性细胞指数均有非常显著性差异（P＜0.01）。增厚期ICE阳性表达率较成熟期显著降低（P＜0.01），而Bcl-2阳性率却显著高于成熟期组（P＜0.01）。 结论 细胞凋亡减少可能与肥厚性瘢痕的形成有关，ICE和Bcl-2可能参与了肥厚性瘢痕成纤维细胞和血管内皮细胞凋亡的调控。
Objective To investigate the relationships between the development of hypertrophic scar(HS) and apoptosis of fibroblast and endothelial cell, and to elucidate the influence of related modulating factors. Methods TUNEL and immunohistochemistry staining techniques were employed to examine apoptosis of fibroblast and endothelial cell.The expression of ICE and Bcl-2 in hypertrophic scar of 61 cases of postburn patients undergoing plastic surgery with HS and in 20 other operative patients with non-hypertrophic scars(NHS)was also determined. Results The positive apoptotic cell indices of fibroblast and vascular endothelial cells during the proliferative stage of HS were 6.60±4.43 and 8.90±6.01 respectively,while those during the mature stage were 25.60±5.70 and and 26.6±6.02, respectively.There existed evident differences in positive apoptotic cell indices between the same kind of cells (fibroblasts or vascular endothelial cells) during the two stages(P＜0.01). The ICE positive expression rate in proliferative stage was much lower than that in mature stage (P＜0.01),but the Bcl-2 positive expression rate in proliferative stage was obviously higher than that in mature stage(P＜0.01). Conclusion HS development might be related to the decrement of apoptotic fibroblasts and endothelial cells.ICE and Bcl-2 were possibly involved in the modulation of fibroblast and endothelial cell apoptosis in HS.
出处
《中华烧伤杂志》
CAS
CSCD
2001年第1期25-28,共4页
Chinese Journal of Burns
