摘要
利用大鼠肝再生增强因子（ａｕｇｍｅｎｔｅｒｏｆｌｉｖｅｒｒｅｇｅｎｅｒａｔｉｏｎ，ＡＬＲ）核苷酸序列，从人胎肝ｃＤＮＡ文库中筛选到人肝再生增强因子ｃＤＮＡ克隆，该克隆含有１．５ｋｂ的外源插入片段，核苷酸序列测定表明含３７５ｂｐ的读码框架，能编码１２５个氨基酸的蛋白质；与大鼠肝再生增强因子相比，核苷酸序列同源性达８７％，氨基酸序列同源性达８４．８％。
In order to isolate cDNA encoding complete human augmenter of liver regeneration (ALR), the strategy of screening an arrayed human fetal cDNA library by PCR was adopted. Three positive clones obtained after 10 6 recombinants from the human fetal liver cDNA library were screened. The sequence analysis showed that these clones covered the coding region of human ALR, with 87% homology to rat ALR.
出处
《军事医学科学院院刊》
CSCD
北大核心
1996年第4期241-244,共4页
Bulletin of the Academy of Military Medical Sciences
基金
国家863计划资助项目
