摘要
目的克隆人肝再生增强因子（ａｕｇｎｅｎｔｅｒ ｏｆ ｌｉｖｅｒ ｒｅｇｒｎｅｒａｔｉｏｎ， ＡＬＲ）的基因组ＤＮＡ，并进行序列分析。方法采用人肝再生增强因子ｃＤＮＡ及其编码序列，对基因的核苷酸序列数据库（ＧｅｎＢａｎｋ）以Ｂｌａｓｔ为工具进行核苷酸同源性比较分析，寻找相应的ＡＬＲ基因组ＤＮＡ序列。结果从ＧｅｎＢａｎｋ核苷酸序列数据库中寻找到人ＡＬＲ基因组ＤＮＡ全序列，由１８１３个核苦酸组成。人ＡＬＲ基因组ＤＮＡ序列由３段外显子 （ｅｘｏｎ）组成，分别为１８ｎｔ、１９７ｎｔ和１６３ｎｔ。基因的编码产物由１２５个氨基酸残基组成，与小鼠ＡＬＲ基因组ＤＮＡ结构类似。基因组ＤＮＡ定位于染色体的１６ｐ１３．３位点上。结论获得人ＡＬＲ基因组ＤＮＡ全序列克隆。
Objective To clone the human genomic DNA of augmenter of liver regeneration(ALR) and specify the nitron-exon structure. Methods Using human ALR cDNA sequence as a reference and BLAST path as a nucleotide homology search tool, GenBank has been searched for ALR homologous genomic DNA sequence. The nitron-exon sequences were defined by the Breathnath-Chambon rule. Results The coding sequence of human ALR consists of 3 e-cons, and is similar to murine ALR genomic DNA structure. The human ALR genomic DNA is 1 813 nt long and codes a protein of 125 amino acid residues. Conclusion Human genomic DNA of ALR consists of 3 e-cons and 2 nitrons.
出处
《中华肝脏病杂志》
CAS
CSCD
2000年第1期12-14,共3页
Chinese Journal of Hepatology
关键词
克隆
肝再生增强因子
肝再生
DNA
Liver
Cloning, molecular
Augmenter of liver regeneration
