摘要
将鸡胚成纤维细胞 ( CEF)分别与当归多糖 ( CAPS)、黄芪多糖 ( APS)、板蓝根多糖 ( IRPS)、淫羊藿多糖 ( EPS)、蜂胶多糖 ( PPS)、淫羊藿黄酮 ( EF)、蜂胶黄酮 ( PF)、黄芪黄酮 ( AF)、黄芪皂甙 ( AS)和人参皂甙 ( GS)等 10种中药成分一起加入到 96孔细胞培养板中培养 ,分别在培养 2 4、36、4 8、6 0、72 h时用 MTT法测定中药成分对 CEF增殖的影响。结果表明 ,所有中药成分均能显著促进 CEF增殖 。
Chick embryo fibroblast(CEF) were cultured together respectively with ten Chinese herbal medicinal ingredients,Chinese angelica polysaccharide(CAPS),astragalus polysaccharide(APS),isatis root polysaccharide(IRPS),epimedium polysaccharide(EPS),propolis polysaccharide(PPS),epimedium flavone(EF),propolis flavone(PF),astragalus flavone(AF),astragalus saponin(AS) and ginsenoside(GS).At 24,36,48,60 and 72 hours after cultivation,their effects on CEF proliferation were assayed by MTT methods.The results showed that all of them at suitable concentration could stimulate proliferation of the cell and there were certain dose and time effect relationships.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2003年第6期601-603,共3页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目 (3 0 0 70 5 66)
