摘要
目的建立补阳还五汤中毛蕊异黄酮苷、芍药苷、羟基红花黄色素A的含量测定方法。方法采用高效液相色谱法,色谱柱:Kromasil C18(250mm×4.6mm,5μm);流动相:甲醇-乙腈(26∶2)为流动相A,0.7%磷酸为流动相B,梯度洗脱;检测波长:毛蕊异黄酮苷为260nm,芍药苷为230nm,羟基红花黄色素A为403nm;柱温:35℃。结果毛蕊异黄酮苷在0.0539～1.0780μg范围内线性关系良好,r=0.9995,平均回收率为99.4%,RSD=1.5%;芍药苷在0.4160～8.3200μg范围内线性关系良好,r=0.9998,平均回收率为100.6%,RSD=1.7%;羟基红花黄色素A在0.0418～0.8352μg范围内线性关系良好,r=0.9995,平均回收率为101.0%,RSD=1.9%。结论本方法简单快捷,适用于测定补阳还五汤中毛蕊异黄酮苷、芍药苷、羟基红花黄色素A的含量。
Objective To establish a HPLC method for the simultaneous determination of campanulin, paeoniflorin and hydroxysafflor yellow A in Buyang Huanwu Decoction. Methods A Kromasil C18（4.6 mm× 250 mm, 5 μm） column was adopted. The mobile phase consisited of methanol and acetonitrile（26 ： 2） （A）, and 0.7 % phosphoric acid solution（B）, in gradient elution （0 - 10 min, 25 %→35 % A; 10 - 25 min, 35 %→40 % A）. The flow rate was at 1.0 mL · min^-1. The column temperature was 35 ℃, and the detection wavelengths were set at 260nm for campanulin, 230 nm for paeoniflorin and 403 nm for hydroxysafflor yellow A. Results The linear ranges of campanulin, paeoniflorin and hydroxysafflor yellow A were 0. 0539 1. 078 μg （ r = 0. 999 5）, 0. 4160 - 8. 320 μg （ r = 0. 999 8）, and 0. 0418 - 0. 8352 μg （ r = 0. 999 5） respectively; the average recovery was 99.4 % （RSD = 1.5 % ）, 100.6 % （RSD = 1.7 % ）, and 101.0 % （RSD = 1.9 % ） respectively. Conclusion The method is simple, feasible and reproducible, and can be used for the quality control of Buyang Huanwu Decoction.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2009年第4期373-376,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
