摘要
将 5种浓度的含当归多糖 (CAPS)、黄芪多糖 (APS)、板蓝根多糖 (IRPS)、淫羊藿多糖 (EPS)、蜂胶多糖 (PPS)、淫羊藿黄酮 (EF)、蜂胶黄酮 (PF)、黄芪黄酮 (AF)、黄芪皂甙 (AS)和人参皂甙 (GS) 1 0种中药成分分别加入到已培养 48h、 1 2h前接种新城疫病毒的鸡胚成纤维(CEF)细胞单层中 ,于病毒接种后 72h用中性红染料吸收法测定CEF细胞的活性 ,以评价各中药成分对培养细胞的生长和抗病毒感染的影响。结果表明 ,EF、PPS和PF显著促进细胞生长 ,CAPS、IRPS和GS显著抑制细胞生长 ,EF、GS、PPS、AF、PF、APS和EPS 7种中药成分显著抑制病毒感染 ;部分中药成分的作用各有所长 ,且有一定的量效关系。
Ten Chinese herbal medicinal ingredients,Chinese angelica polysaccharide(CAPS),astragalus polysaccharide(APS),isatis root polysaccharide(IRRS),epimedium polysaccharide(EPS),propolis polysaccharide(PPS),epimedium flavone(EF),propolis flavone(PF),astragalus flavone(AF),astragalosides(AS)and ginsenosides(GS),at five concentration,were respectively added into the medium for culturing Chick embryo fibroblast(CEF)cels at 12 hours after inoculating with Newcastle disease virus(NDV).At 72 hours post-inoculation,the cellular activity was tested by Neutral Red Assay to evaluate the effects of the ten ingredients on the growth and resisting viral infection of the cell.The results showed that EF,PPS and PF promoted the cell growth and CAPS,IRPS and GS restrained the cell growth.EF?AS?PPS?AF?PF?APS and EPS inhibited the virus infection significantly.In addition,there were certain dose-effect relationships.
出处
《畜牧与兽医》
北大核心
2003年第11期4-7,共4页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金资助项目 (30 0 70 566)。
