摘要
背景与目的:近年的研究发现,一氧化氮在恶性肿瘤的发生和发展中起着重要作用,且在正常骨细胞中检测到一氧化氮合酶(nitric oxide synthase,NOS)的活性,但目前未见有关骨巨细胞瘤(giant cell tumors of bone,GCT)NOS的活性及其与GCT病理分级和肿瘤复发关系的报道。本研究探讨GCT组织中NOS mRNA和蛋白的表达,及其与GCT病理分级、肿瘤复发的关系。方法;用NOS的cDNA探针检测14例冻存GCT组织中原生型一氧化氮合酶(constitutive NOS,cNOS)、诱导型一氧化氮合酶(inducible NOS,iNOS)mRNA的表达,用抗NOS1、NOS2、NOS3的多克隆抗体检测42例GCT石蜡包埋组织中NOS1、NOS2、NOS3蛋白的表达。结果:(1)在14例冻存的GCT组织中多核巨细胞(multinuclear giant cell,MGC)cNOS和iNOS mRNA的阳性率分别为78.6％和57.1％,单核细胞(mononuclaer cell,MC)的阳性率均为 35.7％;(2)病理分级Ⅱ、Ⅲ级MGC的cNOS mRNA阳性率明显高于Ⅰ级MGC(P=0.008);(3)在42例GCT的石蜡包埋组织中,MGC中NOS1、NOS2、NOS3蛋白的阳性率分别为85.7％、59.5％和31.0％,MC的阳性率分别为54.8％、28.6％和14.3％;(4)病理分级Ⅱ、Ⅲ级CCT组织中MC的NOS1阳性率明显高于Ⅰ级组(P=0.006);(5)复发组GCT组织MC的NOS1阳性率明显高于无复发组(P=0.018)。
BACKGROUND & OBJECTIVE: Recent studies have indicated that nitric oxide (NO) plays an important role in carcinogenesis and tumor progression. Activity of nitric oxide synthase (NOS) has been detected in normal bone cell lines. There was no report about relation of expression of NOS in giant cell tumors(GCT) of bone with its pathological grading and tumor recurrence. This study was designed to investigate the relationship amoung expression of NOS mRNAs, NOS proteins, pathological grading and tumor recurrence. METHODS: In situ hybridization (ISH) with cDNA probe was used to determine 14 frozen GCT specimens for constitutive NOS(cNOS) mRNA and inducible NOS(iNOS) mRNA. Immunohistochemical (IHC) staining with multiclonal antibodies was used to determine 42 paraffin-embedded GCT specimens for protein expression of NOS1, NOS2, and NOS3. RESULTS: (1) In 14 frozen GCT specimens, the positive expression rates of cNOS and iNOS mRNAs of multinuclear giant cells (MGC) were 78.6% and 57. 1% ; the positive expression rates of cNOS and iNOS mRNAs in mononuclear cells (MC) were both 35.7%. (2) The positive expression rate of cNOS mRNA in MGC of groups grading Ⅱ and Ⅲwas significantly higher than that of group grading I (P =0.008). (3) In 42 paraffin-embedded GCT specimens, the positive expression rates of NOS1, NOS2, and NOS3 protein were 85. 7%, 59.5%, and 31.0% in MGC, 54.8%, 28.6%, and 14.3% in MC, respectively. (4) The positive expression rate of NOS1 protein in MC of groups grading Ⅱ, Ⅲ was significantly higher than that of group grading I ( P = 0. 006). (5)The positive expression rate of NOS1 protein in MC of the recurrent group was significantly higher than that of the non-recurrent group (P = 0. 018) . The positive expression rate of NOS3 protein in MGC of recurrent group was significantly higher than that of the non-recurrent group ( P = 0. 041). CONCLUSION: The expression of NOS in GCT, especially cNOS in MC, is closely related to the pathological grading and the recurrence of GCT.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2003年第11期1180-1183,共4页
Chinese Journal of Cancer
基金
广东省自然科学基金(No.97073)
关键词
一氧化氮合酶
MRNA表达
蛋白
骨巨细胞瘤
肿瘤复发
Bone neoplasms
Nitric oxide synthase (NOS)
In situ hybridization (ISH)
Immunohistochemistry
