摘要
目的探讨ｎｍ２３Ｈ１基因与恶性及半恶性骨肿瘤相关性。方法以３０例骨肿瘤手术切除组织为标本，分别进行ｎｍ２３Ｈ１ｍＲＮＡ原位杂交及单克隆抗体的免疫组化研究。结果ｎｍ２３Ｈ１基因的表达在骨巨细胞瘤４例原发者中为阳性，３例复发者中为阴性；在８例骨肉瘤中６例阳性；在Ｅｗｉｎｇ瘤、恶性纤维组织细胞瘤及转移癌中表达基本为阴性；在４例软骨肉瘤中３例阴性。结论ｎｍ２３Ｈ１的表达可能与骨巨细胞瘤的复发有一定相关性，而在骨肉瘤的研究中未发现其相关性。
Objective To explore the correlation between nm23H1 gene and malignant and semi-malignant bone tumors. Method Thirty surgical specimens were collected,including 8 osteosarcomas,7 giant cell tumors,4 chondrosarcomas,2 Ewings sarcomas,2 malignant fibrohistocytomas,1 neurofibrosarcoma,1 schwannoma,and 5 metastatic cancers.The expression of nm23H1 in these specimens was observed by in situ hybridization and immunohistochemical staining. Result The expression of nm23H1 in 4 cases of primary giant cell tumor (GCT) was high,but was low or absent in 3 recurrent ones.In 4 cases of chondrosarcomas, the expression was positive.Its expression was basically negative in Ewings tumor,malignant fibrous histiocytoma and metastatic sarcomas. Conclusion There is no association of nm23H1 with osteosarcoma.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1998年第8期477-479,I093,共4页
Chinese Journal of Surgery
基金
国家自然科学基金
关键词
基因表达
骨肿瘤
原位杂交
NM23-H1基因
Gene expression Bone neoplasms In situ hybridization Immunohistochemistry
