摘要
根据猪传染性胃肠炎病毒 (TGEV)和猪呼吸道冠状病毒 (PRCV)的基因组核苷酸序列 ,在S基因 (纤突蛋白基因 ) 5′端保守区设计了一对引物P3 /P4,该对引物在TGEV扩增跨幅约为 2 .4kb ;而PRCV由于在此区域存在一约 0 .6kb碱基缺失 ,扩增跨幅约为 1.8kb。用引物P3 /P4对TGEVMiller株、Purdue株和PRCVAR310株分别进行RT_PCR ,根据RT_PCR扩增片段大小可以直接区分TGEV和PRCV。用引物P3 /P4与引物P1/P2 作Nested_PCR ,提高了该RT_PCR的特异性和敏感性 。
According to the published sequence of TEGV and PRCV,one pair of specific primers were designed as P 3/P 4 from S gene of TGEV and PRCV.The region between the primers were about 2.4kb of TGEV,and 1.8kb of PRCV,which PRCV has an deletion 0.6kb on s gene.The Pair of primers P 3/P 4 was used to detect 3 reference strains (TGEV Miller strain,purdue strain and PRCV AR310 strain) by RT_PCR.All strains Produced the predicted products.Based on the size of RT_PCR products,TGEV and PRCV could be quickly and easily discriminated. Nested_PCR with Primers P 3/P 4 and P 1/P 2 could improve the specificity and sensitivity.The RT_PCR may be used to detect TGEV and PRCV and distinguish them.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2002年第4期301-303,共3页
Chinese Journal of Preventive Veterinary Medicine
