摘要
目的:探讨中药脑溢安颗粒对脑出血后神经干细胞反应的影响。方法:72只雄性SD大鼠单纯随机分为假手术组、模型组、脑溢安组,采用Rosenberg方法复制大鼠脑出血模型,分别给予不同处理,于术后1,12h,1,3,5,7d处死动物,运用免疫组织化学,Westernblot方法检测脑出血后各组的nestin表达。结果:脑出血后,nestin阳性细胞增多,1d达高峰,然后逐渐降低,维持到第7天,nestin阳性细胞主要分布在海马、皮质区,且脑溢安组与模型组1d前各时间点差异无显著性意义(P>0.05),3d后各时间点差异有显著性意义(t分别为3.081,8.034,5.833,P<0.05～0.01);nestin蛋白表达有相同趋势。结论:正常脑组织中存在一定数量的神经干细胞,脑出血后神经干细胞被激活增殖,中药脑溢安能维持促进增殖,这种作用可能是通过调节细胞外因子来实现。
AIM: To investigate the effect of NaoYiAn(NYA)granules on proliferation of n eural stem cells after intracerebral hemorrhage. METHODS: Seventy-two male SD rats were randomly divided into shame operate gr oup, model group and Naoyian group.There were 24 rats in each group. The three g roups were given different treatment.Five healthy rats were used as normal contr ols.Cerebral hemorrhage model of rats was established according to Rosenberg's m ethods.Four rats of each group were killed at 1 and 12 h, 1, 3, 5 and 7 d respec tively. Western blot and immunohistochemistry were used to detect nestin express ion in the former three groups following cerebral hemorrhage. RESULTS: After intracerebral hemorrhage,the number of nestin positive cells in creased,reached the peak at 1 d,and maintained until the 7th day.These cells wer e scattered in hippocampus and cortex.Differences in the number of nestin positi ve cells between NYA group and model group were significant at 3,5 and 7 d respe ctively after intracerebral hemorrhage (t=3.081,8.034,5.833,P< 0.05-0.01),but i nsignificant at 1 and 24 h after intracerebral hemorrhage (P >0.05).
出处
《中国临床康复》
CSCD
2003年第25期3428-3429,T001,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助(30171192)~~
关键词
脑出血
神经干细胞
细胞增殖
脑溢安颗粒
中药制剂
There are neural stem cells in normal brain,and after intracerebral hemorrhage neural stem cells are activated and begin to proliferate.NaoYiAn is helpful to maintain and promote the proliferation of cells as a result of the re gulation of extr
