摘要
目的 :探讨低温冻存对脐血CD34+ 细胞的影响。方法 :采用流式细胞仪分析冻存前后脐血CD34+细胞百分率、CD4 5 + 细胞和CD34+ 细胞的荧光强度变化及死细胞群的分布情况。结果 :冻存后CD34+ 细胞占CD4 5 + 细胞的百分率 [(0 .84± 0 .39) % ]明显高于冷冻前 [(0 .5 1± 0 .2 4 ) % ](P <0 .0 1) ,冻存前后CD34+ 细胞绝对数无明显变化 [(9.372± 6 .0 72 )× 10 6/L和 (9.2 4 6± 6 .132 )× 10 6/L](P >0 .0 5 ) ,冻存前后CD34+ 细胞百分率呈正直线相关 (r =0 .5 6 4 ,P <0 .0 1)。冻存后CD4 5 + 细胞荧光强度减弱 (P <0 .0 1) ,CD34+ 细胞荧光强度无明显变化 (P >0 .0 5 ) ;中性粒细胞比例下降 ,淋巴细胞和单核细胞比例增高。死细胞组分中以中性粒细胞为主 ,占 81.5 2 % ;活细胞组分中以淋巴细胞为主 ,占 5 9.4 4 %。结论 :冻存后CD34+ 细胞占CD4 5 + 细胞的百分率增高 ,但低温冻存对CD34+ 细胞绝对数量影响不大。死细胞主要为较成熟的粒细胞 ,冻存后CD34+ 细胞的分析需排除死细胞的干扰。
Objective:To study the effect of cryopreservation on cord blood CD34 + cells. Method:The percentage of CD34 + cells before and after cord blood cryopreservation were tested by flow cytometry(FCM). The percentage of CD34 + cells,the change of fluorescence intensity for CD45 + cells and CD34 + cells and the distribution ratio of dead cells group were analyzed before and after cryopreservation. Result:The percentage of CD34 + cells in CD45 + nucleated cells after cord blood cryopreservation was significantly highter than in fresh [( 0.84± 0.39)% versus ( 0.51± 0.24)%,P< 0.01],but the absolute count of CD34 + cells change little,[( 9.372± 6.072) ×10 6/L versus ( 9.246± 6.132)×10 6/L,P> 0.05].There was positive linear correlation between the percentage of CD34 + cells before and after cryopreservation(r= 0.564, P< 0.01). The fluorescence intensity of CD45 + cells were weaken(P< 0.01)and that of CD34 + cells didn't change significantly(P> 0.05). The percentage of lymphocytes and monocytes increased and that of neutrophils decreased after cryopreservation. The majority of dead cells were neutrophils, which was 81.52% of 7-AAD +/ CD45 + and the percentage of lymphocytes in 7-AAD -/CD45 + living cells was 59.44%. Conclusion:The percentage of CD34 + cells in CD45 + nucleated cells after cryopreservation increased, but there was no evident influence of cryopreservation on CD34 + cells. Major dead cells were mature granulocytes.Analysis of CD34 + cells after cryopreservation were needed to exclude dead cells.
出处
《临床血液学杂志》
CAS
2003年第6期257-259,共3页
Journal of Clinical Hematology
基金
广东省科委课题
广东省卫生厅"五个一工程"重点课题 (No 96 Z 6 4 1和 99 Z 0 0 8 0 1)
