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丝状真菌表达系统在thaumatin基因工程中的优化 被引量：1

Optimization of Expression System Of Filamentous Fungi in Thaumatin Genetic Engineering
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摘要 　在thaumatin重组生产中,丝状真菌是最成功的表达系统,十几年来,为了最大程度地获得重组thaumatin,人们对丝状真菌表达系统进行了各种优化,如选用特异性强启动子、增加基因拷贝数、利用蛋白酶缺陷株、进行基因融合、优化反应条件等。随着丝状真菌表达系统的优化,重组thaumatin的产量也随之提高。 Filamentous fungi are attractive hosts for the expression of the recombinant thaumatin. People have optimized the expression system of filamentous fungi for the production of thaumatin at high concentration. The specifically strong promoters, the genetic copy numbers, the proteasedeficient mutants, gene fusion and the optimization of the culture conditions are needed to the optimization of the expression system of filamentous fungi. The yields of recombinant thaumatin have improved with the optimization of the expression system in the filamentous fungi.
作者 裴凌鹏 孔建强 赵琦
机构地区 北京联合大学应用文理学院 首都师范大学生物系
出处 《北京联合大学学报》 CAS 2003年第3期75-77,共3页 Journal of Beijing Union University
关键词 丝状真菌 表达系统 基因工程 蛋白酶 甜味剂 Filamentous fungi expression system promoter optimization
分类号 TS202.3 [轻工技术与工程—食品科学] Q786 [生物学—分子生物学]
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参考文献6

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二级参考文献3

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共引文献5

同被引文献35

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引证文献1

二级引证文献4

北京联合大学学报
2003年 第3期

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