摘要
将克隆在不同质粒上的thaumatin基因的两个片段连接起来 ,连接产物克隆到质粒pUC18上 .测序结果表明 ,连接产物是一个完整的thaumatincDNA基因 .将此基因通过基因重组技术 ,克隆到植物表达载体pBI12 1中 ,构建了一个新的转化植物的表达载体—pBI12 1 tha.
After cheated with T\-4 ligase, the two fragments which were cloned into two different plasmids were ligated into a complete thaumatin gene. The traget gene was confirmed by sequencing. By DNA recombinatnt methods, the cDNA gene was cloned into vector pBI121.A new expressing vector\_\_pBI121\|tha was successfully constructed.
出处
《首都师范大学学报（自然科学版）》
2003年第2期64-67,共4页
Journal of Capital Normal University：Natural Science Edition
基金
国家自然科学基金资助 (A3 0 0 70 488)
