摘要
目的:研究二烯丙基二硫(DADS)对人胃癌MGC803细胞生长的抑制作用。方法:采用MTT法、生长曲线分析、细胞活力检测、双层软琼脂集落形成率、及倒置显微镜等方法,观察DADS对体外培养的人胃癌MGC803细胞的影响。结果:DADS对MGC803细胞具有明显的生长抑制效应,且呈剂量-效应依赖关系(P<0.05)。培养96 h,35mg/LDADS的抑制作用呈时间-效应依赖关系(P<0.05)。细胞群体倍增时间由33.8 h处长至DADS处理后的84.0 h(P<0.05);细胞存活率分别为阴性对照组97.4％和DADS处理组80.4％(P>0.05);软琼脂集落形成率由 1.23％下降到0.33％(P<0.05)。阴性对照组细胞多角形、圆形,体积大,多形性明显,核大小不一,见双核及核仁,细胞生长紧密呈堆叠生长,DADS处理后细胞异型性降低,为形态一致的梭形,体积小,境界清楚而分散,胞质丰富,核明显变小。结论:DADS对体外培养的MGC803细胞具有明显的增生抑制作用,且呈现剂量-效应依赖关系。
AIM: To investigate the effect of diallyl disulfide(DADS) on human gastric cancer MGC803 cell line in vitro. METHODS: The effect of DADS was confirmed by MTT assay, cell growth curve analysis, cell viability detection, clony formation in soft agar and inversion microscopy. RESULTS: In vitro growth of MGC803 cells was significantly inhibited by DADS in a dose-dependent manner (P<0.05) when MGC803 cells were cultured for 96 hours, and at a higher concentration of 35 mg/L. The inhibiting effect of DADS displayed a time-dependent manner (P<0.05). Cell viability decreased from 97.4 % in negative control group to 80.4 % in treatment group. There was no significance existed between the two groups (P>0.05), and average doubling time was delayed from 33.75 h in negative control group to 84.0 h in treatment group (P<0.05).Clony for- mation ratio was also decreased from 1.23 % in negative control group to 0.33% in treatment group (P<0.05). After treated by DADS, MGC803 cells showed plemorphic and atypia declining, small size, uniform spindle shape, abun- dant cytoplasm and smaller nuclei.All these appeared in 20 mL/L DMSO treated MGC803 cells as well as in DADS- expose cells. CONCLUSION: The effect of growth inhibition of DADS on human gastric cancer MGC803 cells in vitro is Signifecant and exhibits a dose-dependent manner.
出处
《世界华人消化杂志》
CAS
2003年第9期1290-1293,共4页
World Chinese Journal of Digestology
基金
湖南省科委基金
No.97610319
No.00SSY3019
湖南省自然科学基金
No.01JJY2146
No.02JJY2026
