摘要
本文确立下了测定食品中痕量铜(Ⅱ)的催化荧光分析新方法。该方法建立在pH8.8～9.2氨—氯化铵缓冲体系中,铜(Ⅱ)对罗丹明B(RhB)和过氧化氢(H2O2)的反应有强烈的催化作用并使其荧光熄灭的基础上。方法简便快速,具有极高的灵敏度,检出限达4×10-12g/ml,线性范围为10-11～10-6g/ml,且选择性好。用于五类食品中微量铜(Ⅱ)的测定与AAS相符,RSD(n=5)<5%,回收率在94.1%～103%之间。
This paper illustrated a new method of catalytic fluorescence determination of trace Cu(Ⅱ) in foodstuffs.In pH8.2～9.2 NH4-NH4 Cl buffer medium the reaction of Cu(Ⅱ) to Rh B and H2O2 took strong catalytic effect to quench the fluorescence.Being quick and easy to operate,the method showed extremely high sensitivith,with the detection limit as 4×10-12g/ml and thelinear range as from 10-11 to 10-6g/ml.The reagent was stable with small consumption.The relative standard deviation for determiningtrace Cu(Ⅱ) in the five kinds of foodstuffs was less than 5%,with a recovery rate between 94.1% to 103%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2003年第9期116-119,共4页
Food Science
